Division of Neurological Rehabilitation, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
Neural Plast. 2021 Dec 7;2021:1469339. doi: 10.1155/2021/1469339. eCollection 2021.
Functional prognosis is potentially correlated with gut microbiota alterations following the dysregulation of the gut-microbiota-brain axis after stroke. This study was designed to explore the poststroke alterations of gut microbiota and potential correlations between gut microbiota and global functions.
A total of thirty-eight patients with stroke and thirty-five healthy demographics-matched controls were recruited. Their fecal DNAs were extracted, and the V3-V4 regions of the conserved bacterial 16S RNA were amplified and sequenced on the Illumina MiSeq platform. Microbial composition, diversity indices, and species cooccurrence were compared between groups. Random forest and receiver operating characteristic analysis were used to identify potential diagnostic biomarkers. Relationships between discriminant bacteria and poststroke functional outcomes were estimated.
Higher alpha diversity of gut microbiota was observed in poststroke patients as compared to the healthy controls ( < 0.05). Beta diversity showed that microbiota composition in the poststroke group was significantly different from that in the control group. Relative abundance of nine genera increased significantly in poststroke patients, while 82 genera significantly decreased ( < 0.05). The accuracy, specificity, and susceptibility of the optimal model consisted of the top 10 discriminant species were 93%, 100%, and 86%, respectively. Subgroup analysis showed that bacterial taxa abundant between subacute and chronic stroke patients were overall different ( < 0.05). The modified Rankin scale (mRS) ( = -0.370, < 0.05), Fugl-Meyer assessment (FMA) score ( = 0.364, < 0.05), water swallow test (WST) ( = 0.340, < 0.05), and Barthel index (BI) ( = 0.349, < 0.05) were significantly associated with alterations of distinctive gut microbiota.
The gut microbiota in patients with stroke was significantly changed in terms of richness and composition. Significant associations were detected between alterations of distinctive gut microbiota and global functional prognosis. It would facilitate novel treatment target selection in the context of stroke while the causal relationships between distinctive gut microbiota alterations and functional variations need to be further verified with well-designed studies.
功能性预后可能与中风后肠道微生物群-脑-肠轴失调相关的肠道微生物群改变有关。本研究旨在探讨中风后肠道微生物群的变化以及肠道微生物群与整体功能之间的潜在相关性。
共纳入 38 例中风患者和 35 例健康对照组。提取粪便 DNA，采用 Illumina MiSeq 平台扩增和测序细菌 16S RNA 的 V3-V4 区。比较两组间微生物组成、多样性指数和种间共现。采用随机森林和受试者工作特征分析鉴定潜在的诊断生物标志物。评估判别细菌与中风后功能结局的关系。
与健康对照组相比，中风患者的肠道微生物群 α 多样性较高（<0.05）。β 多样性显示，中风组的微生物群落组成与对照组有显著差异。中风患者 9 个属的相对丰度显著增加，而 82 个属的相对丰度显著降低（<0.05）。由前 10 个判别种组成的最佳模型的准确性、特异性和敏感性分别为 93%、100%和 86%。亚组分析显示，亚急性期和慢性期中风患者之间丰富的细菌类群总体不同（<0.05）。改良 Rankin 量表（mRS）（=-0.370，<0.05）、Fugl-Meyer 评估（FMA）评分（=0.364，<0.05）、饮水吞咽试验（WST）（=0.340，<0.05）和 Barthel 指数（BI）（=0.349，<0.05）与独特肠道微生物群的改变显著相关。
中风患者的肠道微生物群在丰富度和组成上均发生显著改变。独特肠道微生物群的改变与整体功能预后之间存在显著相关性。这有助于在中风背景下选择新的治疗靶点，而独特肠道微生物群改变与功能变化之间的因果关系需要进一步通过精心设计的研究来验证。
