The First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China.
College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China.
Front Cell Infect Microbiol. 2022 Jun 14;12:873096. doi: 10.3389/fcimb.2022.873096. eCollection 2022.
Buyang Huanwu decoction (BHD) is a well-known Chinese herbal prescription. It has been widely used in the clinical treatment of cerebral ischemia (CI) in China. However, the mechanism underlying the treatment of CI with BHD remains to be elucidated. In this study, we combined microbiomic and metabolomic strategies to explore the therapeutic effects of BHD on middle cerebral artery occlusion (MCAO) in rats. Our results showed that BHD could effectively improve neurological severity scores and alleviate neuronal damage in rats with MCAO. BHD could also reduce the level of peripheral proinflammatory cytokines and inhibit neuroinflammation. 16S rRNA sequencing showed that BHD could increase the relative abundances of the genera , , , etc., while decreasing the relative abundances of the genera , , etc. Untargeted metabolomic analysis of hippocampal samples showed that 17 significantly differentially abundant metabolites and 9 enriched metabolic pathways were linked with BHD treatment. We also found that the regulatory effects of BHD on metabolites were correlated with the differentially abundant microbial taxa. The predicted function of the gut microbiota and the metabolic pathway enrichment results showed that purine metabolism, glutamatergic synapses, arginine and proline metabolism, and alanine, aspartic acid and glutamate metabolism were involved in the effects of BHD. These pathways may be related to pathological processes such as excitotoxicity, neuroinflammation, and energy metabolism disorder in CI. In summary, these findings suggest that regulation of hippocampal metabolism and of the composition and function of the gut microbiota may be important mechanisms underlying the effect of BHD in the treatment of CI.
补阳还五汤（BHD）是一种著名的中药方剂，在中国临床上广泛用于治疗脑缺血（CI）。然而，BHD 治疗 CI 的机制仍需阐明。在这项研究中，我们结合微生物组学和代谢组学策略来探索 BHD 对大鼠大脑中动脉闭塞（MCAO）的治疗作用。我们的结果表明，BHD 能有效改善 MCAO 大鼠的神经严重程度评分并减轻神经元损伤，还能降低外周促炎细胞因子水平并抑制神经炎症。16S rRNA 测序显示，BHD 能增加属、属、属等的相对丰度，同时降低属、属等的相对丰度。海马样本的非靶向代谢组学分析显示，有 17 个显著差异丰度代谢物和 9 个富集代谢途径与 BHD 治疗相关。我们还发现，BHD 对代谢物的调节作用与差异丰度微生物类群相关。肠道微生物群的预测功能和代谢途径富集结果表明，嘌呤代谢、谷氨酰胺能突触、精氨酸和脯氨酸代谢以及丙氨酸、天冬氨酸和谷氨酸代谢与 BHD 的作用有关。这些途径可能与 CI 中的兴奋性毒性、神经炎症和能量代谢障碍等病理过程有关。综上所述，这些发现表明调节海马代谢以及肠道微生物群的组成和功能可能是 BHD 治疗 CI 的重要机制。
