Chaichompoo Waraluck, Rojsitthisak Pornchai, Pabuprapap Wachirachai, Siriwattanasathien Yuttana, Yotmanee Pathumwadee, Suksamrarn Apichart
Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Natural Products for Aging and Chronic Diseases, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Natural Products for Aging and Chronic Diseases, Chulalongkorn University, Bangkok 10330, Thailand.
Phytochemistry. 2024 Jan;217:113929. doi: 10.1016/j.phytochem.2023.113929. Epub 2023 Nov 19.
Eleven previously undescribed Amaryllidaceae alkaloids, crinalatifolines A-K (1-11), and two first naturally occurring alkaloids, dihydroambelline (12) and N-demethyldihydrogalanthamine (13), were isolated from the bulbs of Crinum latifolium L. Additionally, thirty-seven known alkaloids and one alkaloid artifact were also isolated from this plant species. Their structures and absolute configurations were elucidated using extensive spectroscopic techniques, including IR, NMR, MS, and ECD. Evaluations of the cholinesterase inhibitory activities of most of these compounds were conducted. Among the tested compounds, ungeremine exhibited the highest potency against acetylcholinesterase and butyrylcholinesterase, with the IC values of 0.10 and 1.21 μM, respectively. These values were 9.4- and 2.4-fold more potent than the reference drug galanthamine.
从宽叶文殊兰的鳞茎中分离出11种以前未描述过的石蒜科生物碱，即宽叶文殊兰碱A - K（1 - 11），以及两种首次天然存在的生物碱，二氢安贝灵（12）和N - 去甲基二氢加兰他敏（13）。此外，还从该植物物种中分离出37种已知生物碱和1种生物碱衍生物。利用红外光谱（IR）、核磁共振（NMR）、质谱（MS）和电子圆二色谱（ECD）等广泛的光谱技术对它们的结构和绝对构型进行了阐明。对其中大多数化合物的胆碱酯酶抑制活性进行了评估。在测试的化合物中，安格明对乙酰胆碱酯酶和丁酰胆碱酯酶表现出最高的活性，其IC值分别为0.10和1.21 μM。这些值分别比参比药物加兰他敏强9.4倍和2.4倍。
