鲍威尔文殊兰“Album”（石蒜科）的生物碱及其拓扑异构酶抑制活性。
Alkaloids of Crinum x powellii "Album" (Amaryllidaceae) and their topoisomerase inhibitory activity.
作者信息
Niño Jaime, Hincapié Gina M, Correa Yaned M, Mosquera Oscar M
机构信息
Grupo de Biotecnología - Productos Naturales, Escuela de Tecnología Química, Universidad Tecnológica de Pereira, Pereira, Colombia.
出版信息
Z Naturforsch C J Biosci. 2007 Mar-Apr;62(3-4):223-6. doi: 10.1515/znc-2007-3-411.
The alkaloids lycorine, 1-O-acetyllycorine and ismine were isolated from the basic dichloromethane-soluble fraction of Crinum x powellii "Albu m" bulbs. The alkaloid structures were established by physical and spectroscopic analyses, including 1D NMR techniques and GC-MS analysis. The three alkaloids are reported for the first time for this hybrid. Additionally, the three alkaloids isolated were tested against a mechanism-based bioassay utilizing genetically engineered mutants of the yeast Saccharomyces cerevisiae strains RAD+, RAD52Y and RS321 where lycorine was the only alkaloid that displayed moderate topoisomerase I inhibitory activity.
从粉花文殊兰“Album”鳞茎的碱性二氯甲烷可溶部分中分离出了生物碱石蒜碱、1-O-乙酰石蒜碱和水仙环素。通过物理和光谱分析，包括一维核磁共振技术和气相色谱-质谱分析，确定了生物碱的结构。这三种生物碱首次在这种杂交品种中被报道。此外，利用酿酒酵母菌株RAD+、RAD52Y和RS321的基因工程突变体，对分离出的这三种生物碱进行了基于机制的生物测定，其中石蒜碱是唯一显示出中等拓扑异构酶I抑制活性的生物碱。
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