Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, China.
Department of Microbiome, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, China.
Orthod Craniofac Res. 2024 Feb;27(1):174-184. doi: 10.1111/ocr.12733. Epub 2023 Nov 20.
To investigate the salivary bacterial communities during the first 6-month orthodontic treatment with Clear Aligners (CA) and Fixed Appliances (FA), and its correlation with clinical periodontal parameters.
Saliva and periodontal parameters were sampled from individuals wearing CA or FA before treatment (T0), and after 3- (T3) and 6-month (T6) treatments. Salivary bacterial communities characterized based on the 16S rRNA V3-V4 region were compared between FA and CA and correlated with clinical periodontal parameters.
Probing Depth (PD) significantly increased at T6 in the FA group versus T0, whereas it remained stable in the CA group. The Shannon and Pielou indices were significantly higher in the FA group and significantly positively correlated with periodontal inflammation parameters. β-diversity analysis revealed distinct communities between the FA group and CA group at T6. The relative abundances of 3 genera and 15 species were significantly higher in the FA group. Among the above appliance-type related taxa, bacterial genera Selenomonas, Stomatobaculum, Olsenella and Faecalicoccus and bacterial species Selenomonas_sputigena, Dialister_invisus, Olsenella_profus, Prevotella_buccae, Cryptobacterium_curtum and Clostridium_spiroforme were significantly positively associated with periodontal parameters.
Orthodontic treatments trigger appliance-related salivary bacterial communities, highlighting the importance of developing appliance-orientated periodontal strategies during orthodontic treatments. Salivary bacterial communities harboured by patients wearing FA possess higher bacterial parameters which were associated with increasing PD, PI and Gingival Index.
探讨使用 Clear Aligners（CA）和固定矫治器（FA）矫正治疗前 6 个月唾液中的细菌群落及其与临床牙周参数的相关性。
在治疗前（T0）、治疗后 3 个月（T3）和 6 个月（T6）时，从佩戴 CA 或 FA 的个体中采集唾液和牙周参数。根据 16S rRNA V3-V4 区对唾液细菌群落进行特征描述，并比较 FA 和 CA 之间的差异，同时分析其与临床牙周参数的相关性。
与 T0 相比，FA 组在 T6 时探诊深度（PD）显著增加，而 CA 组则保持稳定。FA 组的 Shannon 和 Pielou 指数显著升高，且与牙周炎症参数呈显著正相关。β多样性分析表明，T6 时 FA 组和 CA 组之间存在明显不同的群落。FA 组中 3 个属和 15 个种的相对丰度显著较高。在上述与矫治器相关的分类群中，细菌属 Selenomonas、Stomatobaculum、Olsenella 和 Faecalicoccus 以及细菌种 Selenomonas_sputigena、Dialister_invisus、Olsenella_profus、Prevotella_buccae、Cryptobacterium_curtum 和 Clostridium_spiroforme 与牙周参数呈显著正相关。
正畸治疗会引发与矫治器相关的唾液细菌群落变化，这凸显了在正畸治疗中制定针对矫治器的牙周策略的重要性。佩戴 FA 的患者的唾液细菌群落具有更高的细菌参数，与 PD、PI 和牙龈指数的增加有关。
