Department of Biology, Deanship of Educational Services, Qassim University, P.O. Box 5888, Unaizah 56219, Qassim, Saudi Arabia.
Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University, P.O. Box 44519, Zagazig 44516, Egypt.
Adv Respir Med. 2023 Nov 15;91(6):532-545. doi: 10.3390/arm91060039.
House dust mite (HDM)-allergic asthma is an abnormal immune response to extrinsic aeroallergens found in human vicinities. Studying the role of the associated immunity biomarkers and their interplay helps in discovering novel therapeutic strategies that can be used in adjunct with effective long-term immunotherapy. This study investigates the total serum IgE, FoxO1, and Sirtuin 1 (SIRT1) gene expressions in HDM-allergic asthma patients. We enrolled 40 patients for each of the following three groups: an HV group of healthy volunteers and HDM/AA and HDM/SCIT groups of HDM-allergic asthma patients who did not and who did receive immunotherapy before recruitment in this study, respectively. The results elucidated that total IgE was strikingly elevated in the HDM/AA group and showed little decline in the HDM/SCIT group. Both FoxO1 and SIRT1 gene expressions showed the highest levels in the HDM/SCIT group. There was a negative correlation between total IgE and both FoxO1 and SIRT1 in the HDM/AA group while there was a positive correlation with SIRT1 in the HDM/SCIT group. In conclusion, the interplay of the three immunity biomarkers related to HDM-allergic asthma after the course of immunotherapy treatment suggests further, broader studies on the feasibility of their role as immunity biomarkers in the control and remission of HDM-allergic asthma.
屋尘螨（HDM）过敏哮喘是一种对人体周围发现的外源性空气过敏原的异常免疫反应。研究相关免疫生物标志物的作用及其相互作用有助于发现新的治疗策略，可以与有效的长期免疫疗法联合使用。本研究调查了 HDM 过敏哮喘患者的总血清 IgE、FoxO1 和 Sirtuin 1（SIRT1）基因表达。我们分别招募了以下三组中的 40 名患者：HV 组为健康志愿者，HDM/AA 组和 HDM/SCIT 组为 HDM 过敏哮喘患者，他们在入组前分别未接受和接受免疫治疗。结果表明，HDM/AA 组的总 IgE 显著升高，而 HDM/SCIT 组的总 IgE 略有下降。FoxO1 和 SIRT1 基因表达在 HDM/SCIT 组中均达到最高水平。在 HDM/AA 组中，总 IgE 与 FoxO1 和 SIRT1 呈负相关，而在 HDM/SCIT 组中与 SIRT1 呈正相关。总之，免疫治疗后与 HDM 过敏哮喘相关的三种免疫生物标志物的相互作用表明，进一步广泛研究它们作为控制和缓解 HDM 过敏哮喘的免疫生物标志物的可行性。
