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皮肤屏障的分子组织。

Molecular Organization of the Skin Barrier.

机构信息

Department of Cellular and Molecular Biology (CMB), Karolinska Institutet, Biomedicum, SE-171 77 Stockholm, Sweden.

出版信息

Acta Derm Venereol. 2023 Nov 21;103:adv13356. doi: 10.2340/actadv.v103.13356.

DOI:10.2340/actadv.v103.13356
PMID:37987626
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10680981/
Abstract

Cryo-electron microscopy of vitreous sections allows for investigation directly in situ of the molecular architecture of skin. Recently, this technique has contributed to the elucidation of the molecular organization of the skin's permeability barrier and its stepwise formation process. The aim of this review is to provide an overview of the procedure for cryo-electron microscopy of vitreous sections, its analysis using atomic detail molecular dynamics modelling and electron microscopy simulation, and its application in the investigation of the barrier structure and formation process of the skin.

摘要

玻璃切片的冷冻电子显微镜使我们能够直接原位研究皮肤的分子结构。最近，这项技术有助于阐明皮肤通透性屏障的分子组织及其逐步形成过程。本文综述了玻璃切片冷冻电子显微镜的程序、使用原子细节分子动力学建模和电子显微镜模拟进行分析，以及在研究皮肤屏障结构和形成过程中的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/34c426e6fa61/ActaDV-103-13356-g017.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/8108e97793f4/ActaDV-103-13356-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/b0eed232d895/ActaDV-103-13356-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/7304b9808da1/ActaDV-103-13356-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/4f4db4458200/ActaDV-103-13356-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/56544e1d1690/ActaDV-103-13356-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/c31400b6d233/ActaDV-103-13356-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/801c5fcbf636/ActaDV-103-13356-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/6f204734c22f/ActaDV-103-13356-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/2091561f7f15/ActaDV-103-13356-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/df1a4ad94300/ActaDV-103-13356-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/b4d00ef86afa/ActaDV-103-13356-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/34c426e6fa61/ActaDV-103-13356-g017.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/8108e97793f4/ActaDV-103-13356-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/b0eed232d895/ActaDV-103-13356-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/7304b9808da1/ActaDV-103-13356-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/cb8cfe01335a/ActaDV-103-13356-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/4f4db4458200/ActaDV-103-13356-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/56544e1d1690/ActaDV-103-13356-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/c31400b6d233/ActaDV-103-13356-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/801c5fcbf636/ActaDV-103-13356-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/582e6e865b89/ActaDV-103-13356-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/6f204734c22f/ActaDV-103-13356-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/3e92b1017db4/ActaDV-103-13356-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/f0118fceb71b/ActaDV-103-13356-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/2091561f7f15/ActaDV-103-13356-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/c58e3d4c9c7b/ActaDV-103-13356-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/df1a4ad94300/ActaDV-103-13356-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/b4d00ef86afa/ActaDV-103-13356-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c42/10680981/34c426e6fa61/ActaDV-103-13356-g017.jpg

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本文引用的文献

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The skin barrier: An extraordinary interface with an exceptional lipid organization.皮肤屏障：具有非凡脂质组织的卓越界面。
Prog Lipid Res. 2023 Nov;92:101252. doi: 10.1016/j.plipres.2023.101252. Epub 2023 Sep 4.
2
Understanding Drug Skin Permeation Enhancers Using Molecular Dynamics Simulations.利用分子动力学模拟理解药物皮肤渗透增强剂。
J Chem Inf Model. 2023 Aug 14;63(15):4900-4911. doi: 10.1021/acs.jcim.3c00625. Epub 2023 Jul 18.
3
Secretion Bias of Lamellar Granules Revealed by Three-Dimensional Electron Microscopy.
三维电子显微镜揭示板层颗粒的分泌偏向
J Invest Dermatol. 2023 Jul;143(7):1310-1312.e3. doi: 10.1016/j.jid.2023.03.1674. Epub 2023 Apr 13.
4
Skin permeability prediction with MD simulation sampling spatial and alchemical reaction coordinates.基于 MD 模拟采样空间和化学坐标预测皮肤渗透性。
Biophys J. 2022 Oct 18;121(20):3837-3849. doi: 10.1016/j.bpj.2022.09.009. Epub 2022 Sep 13.
5
Using molecular simulation to understand the skin barrier.利用分子模拟理解皮肤屏障。
Prog Lipid Res. 2022 Nov;88:101184. doi: 10.1016/j.plipres.2022.101184. Epub 2022 Aug 19.
6
The Skin's Barrier: A Cryo-EM Based Overview of its Architecture and Stepwise Formation.皮肤屏障：基于冷冻电镜的结构概述及其逐步形成过程。
J Invest Dermatol. 2022 Feb;142(2):285-292. doi: 10.1016/j.jid.2021.06.037. Epub 2021 Aug 31.
7
Molecular Reorganization during the Formation of the Human Skin Barrier Studied In Situ.在体研究人皮肤屏障形成过程中的分子重排。
J Invest Dermatol. 2021 May;141(5):1243-1253.e6. doi: 10.1016/j.jid.2020.07.040. Epub 2020 Oct 22.
8
State of the art in Stratum Corneum research: The biophysical properties of ceramides.角质层研究的最新进展：神经酰胺的物理特性。
Chem Phys Lipids. 2018 Nov;216:91-103. doi: 10.1016/j.chemphyslip.2018.09.017. Epub 2018 Oct 3.
9
Marked Changes in Lamellar Granule and Trans-Golgi Network Structure Occur during Epidermal Keratinocyte Differentiation.板层颗粒和反式高尔基体网络结构在表皮角质形成细胞分化过程中发生明显变化。
J Invest Dermatol. 2019 Feb;139(2):352-359. doi: 10.1016/j.jid.2018.07.043. Epub 2018 Sep 18.
10
Epidermal Lamellar Granules.表皮板层颗粒
Skin Pharmacol Physiol. 2018;31(5):262-268. doi: 10.1159/000491757. Epub 2018 Aug 15.