使用猴痘病毒A29蛋白测定血清阳性率和体液反应动力学。

Determination of seroprevalence and kinetics of humoral response using mpox virus A29 protein.

作者信息

Cai Jian-Piao, Chu Wing-Ming, Tam Anthony Raymond, Wang Kun, Han Yuting, Chen Lin-Lei, Zhang Xiaojuan, Choi Charlotte Yee-Ki, Cheng Vincent Chi-Chung, Chan Kwok-Hung, Chen Zhiwei, Hung Ivan Fan-Ngai, Fong Carol Ho-Yan, To Kelvin Kai-Wang

机构信息

State Key Laboratory for Emerging Infectious Diseases, Carol Yu Centre for Infection, Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.

Infectious Diseases Division, Department of Medicine, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China.

出版信息

Commun Med (Lond). 2023 Nov 22;3(1):168. doi: 10.1038/s43856-023-00403-9.

DOI:10.1038/s43856-023-00403-9

PMID:37993690

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10665351/

Abstract

BACKGROUND

Mpox virus (MPXV), previously known as monkeypox virus, has spread globally in 2022. An accurate and convenient antibody test is essential for the determination of seroprevalence and for studying immune response after natural infection or vaccination. Most seroprevalence or vaccine studies used either live MPXV (or vaccinia virus [VACV]) or inactivated MPXV (or VACV) culture lysate for serological assays, but MPXV culture can only be performed in biosafety level 3 (BSL-3) facilities. Here, we developed and evaluated an enzyme immunoassay (EIA) based on the MPXV A29 surface envelope protein.

METHODS

We compared the specificity of the MPXV A29, VACV A27, and VACV lysate EIA using serum specimens collected prior to the global spread of MPXV. Next, we performed these EIAs for serum specimens collected from two mpox patients and an MVA-BN vaccine recipient. We also assessed the kinetics of plasmblast and MPXV A29-specific B-cell response.

RESULTS

Using sera collected from different age groups in Hong Kong, we found that most individuals, including those born before 1981 who have received the smallpox vaccine, tested negative using the MPXV A29 protein. MPXV A29-specific antibody could be detected in the serum of mpox patients and an MVA-BN recipient. In a mpox patient, the frequency of plasmablast and MPXV A29-specific B cell peaked on day 8 post-symptom onset and gradually decreased. Finally, we demonstrated that antibodies against the A29 protein can be used for immunofluorescence staining of MPXV-infected cells.

CONCLUSIONS

MPXV A29 protein is suitable for studying the immune response against MPXV infection.

摘要

背景

猴痘病毒（MPXV），以前称为猴天花病毒，于2022年在全球范围内传播。准确便捷的抗体检测对于确定血清流行率以及研究自然感染或接种疫苗后的免疫反应至关重要。大多数血清流行率或疫苗研究使用活的MPXV（或痘苗病毒[VACV]）或灭活的MPXV（或VACV）培养裂解物进行血清学检测，但MPXV培养只能在生物安全3级（BSL-3）设施中进行。在此，我们开发并评估了一种基于MPXV A29表面包膜蛋白的酶免疫测定（EIA）。

方法

我们使用在MPXV全球传播之前收集的血清标本，比较了MPXV A29、VACV A27和VACV裂解物EIA的特异性。接下来，我们对从两名猴痘患者和一名MVA-BN疫苗接种者收集的血清标本进行了这些EIA检测。我们还评估了浆母细胞和MPXV A29特异性B细胞反应的动力学。

结果

使用从香港不同年龄组收集的血清，我们发现大多数个体，包括那些在1981年之前接种过天花疫苗的人，使用MPXV A29蛋白检测呈阴性。在猴痘患者和MVA-BN接种者的血清中可检测到MPXV A29特异性抗体。在一名猴痘患者中，浆母细胞和MPXV A29特异性B细胞的频率在症状出现后第8天达到峰值，然后逐渐下降。最后，我们证明针对A29蛋白的抗体可用于MPXV感染细胞的免疫荧光染色。