Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
College of Basic Medical and Forensic Medicine, Henan University of Science and Technology, Luoyang, 471000, China.
Adv Mater. 2024 Feb;36(6):e2307680. doi: 10.1002/adma.202307680. Epub 2023 Dec 6.
Antibiotic-resistant bacteria pose a global health threat by causing persistent and recurrent microbial infections. To address this issue, antimicrobial nanoparticles (NPs) with low drug resistance but potent bactericidal effects have been developed. However, many of the developed NPs display poor biosafety and their synthesis often involves complex procedures and the antimicrobial modes of action are unclear. Herein, a simple strategy is reported for designing antimicrobial metal-phenolic network (am-MPN) NPs through the one-step assembly of a seeding agent (diethyldithiocarbamate), natural polyphenols, and metal ions (e.g., Cu ) in aqueous solution. The Cu -based am-MPN NPs display lower Cu antimicrobial concentrations (by 10-1000 times) lower than most reported nanomaterials and negligible toxicity across various models, including, cells, blood, zebrafish, and mice. Multiple antimicrobial modes of the NPs have been identified, including bacterial wall disruption, reactive oxygen species production, and quinoprotein formation, with the latter being a distinct pathway identified for the antimicrobial activity of the polyphenol-based am-MPN NPs. The NPs exhibit excellent performance against multidrug-resistant bacteria (e.g., methicillin-resistant Staphylococcus aureus (MRSA)), efficiently inhibit and destroy bacterial biofilms, and promote the healing of MRSA-infected skin wounds. This study provides insights on the antimicrobial properties of metal-phenolic materials and the rational design of antimicrobial metal-organic materials.
耐药细菌通过引起持续和反复的微生物感染对全球健康构成威胁。为了解决这个问题，已经开发出了具有低耐药性但杀菌效果强的抗菌纳米粒子（NPs）。然而，许多已开发的 NPs 显示出较差的生物安全性，其合成通常涉及复杂的步骤，并且抗菌作用模式尚不清楚。在此，报道了一种通过在水溶液中一步组装种剂（二乙基二硫代氨基甲酸盐）、天然多酚和金属离子（例如 Cu）来设计抗菌金属-多酚网络（am-MPN） NPs 的简单策略。基于 Cu 的 am-MPN NPs 的 Cu 抗菌浓度（低 10-1000 倍）低于大多数报道的纳米材料，并且在包括细胞、血液、斑马鱼和小鼠在内的各种模型中几乎没有毒性。已经确定了 NPs 的多种抗菌模式，包括细菌细胞壁破裂、活性氧物质的产生和醌蛋白的形成，后一种途径是确定多酚基 am-MPN NPs 抗菌活性的独特途径。这些 NPs 对耐多药细菌（例如耐甲氧西林金黄色葡萄球菌（MRSA））表现出优异的性能，有效抑制和破坏细菌生物膜，并促进 MRSA 感染皮肤伤口的愈合。本研究提供了关于金属-多酚材料的抗菌特性和抗菌金属有机材料的合理设计的见解。
