Akyer Sahika Pinar, Karagur Ege Rıza, Ata Melek Tunc, Toprak Emine Kilic, Donmez Aysegul Cort, Donmez Baris Ozgur
Department of Anatomy, School of Medicine, Pamukkale University, Kinikli, Str. No. 11, 20160 Denizli, Turkey.
Department of Medical Genetics, School of Medicine, Pamukkale University, Kinikli, Str. No. 11, 20160 Denizli, Turkey.
Curr Issues Mol Biol. 2023 Oct 31;45(11):8755-8766. doi: 10.3390/cimb45110550.
Osteocytes play an important role as regulators of both osteoclasts and osteoblasts, and some proteins that are secreted from them play a role in bone remodeling and modeling. LPS affects bone structure because it is an inflammatory factor, despite verbascoside's potential for bone preservation and healing. Osteocytes may also be involved in the control of the bone's response to immunological changes in inflammatory situations. MLO-Y4 cells were cultured in either supplemented -MEM alone with a low serum to inhibit cell growth or media with LPS (10 ng/mL) and/or verbascoside (50 g/mL) to show the LPS effect. In our research, LPS treatment increased RANKL levels while decreasing OPG and RUNX2 expression. Treatment with verbascoside reduced RANKL expression. In our work, verbascoside increased the expression of OPG and RUNX2. In MLO-Y4 cells exposed to verbascoside, SOD, CAT, and GSH activities as well as the expression levels of bone mineralization proteins like PHEX, RUNX2, and OPG were all elevated.
骨细胞作为破骨细胞和成骨细胞的调节因子发挥着重要作用，它们分泌的一些蛋白质在骨重塑和骨建模中发挥作用。脂多糖（LPS）是一种炎症因子，会影响骨骼结构，尽管毛蕊花糖苷具有保护骨骼和促进愈合的潜力。骨细胞也可能参与控制骨骼在炎症情况下对免疫变化的反应。MLO - Y4细胞在单独添加低血清的 -MEM培养基中培养以抑制细胞生长，或在含有脂多糖（10 ng/mL）和/或毛蕊花糖苷（50 μg/mL）的培养基中培养以显示脂多糖的作用。在我们的研究中，脂多糖处理增加了核因子κB受体活化因子配体（RANKL）水平，同时降低了骨保护素（OPG）和 runt相关转录因子2（RUNX2）的表达。毛蕊花糖苷处理降低了RANKL的表达。在我们的工作中，毛蕊花糖苷增加了OPG和RUNX2的表达。在暴露于毛蕊花糖苷的MLO - Y4细胞中，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽（GSH）的活性以及诸如磷酸调节中性内肽酶（PHEX）、RUNX2和OPG等骨矿化蛋白的表达水平均升高。
