Li Yipeng, Hu Cailin, Song Ruiqi, Yin Zhihui, Wang Lingyun, Shi Lin, Li Wei, Zheng Zhaisheng, Yang Mengfei
Zhejiang Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation, Jinhua Academy of Agricultural Sciences, Jinhua 321000, China.
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
J Fungi (Basel). 2023 Nov 1;9(11):1067. doi: 10.3390/jof9111067.
The is usually infected by the obligate parasitic fungus to form an edible fleshy stem which is an aquatic vegetable called Jiaobai in China. The infection by the teliospore (T) strain of induces forming gray fleshy stems, while the mycelia-teliospore (MT) strain of induces white fleshy stems which are more suitable for edibility than gray fleshy stems. The mechanism of this phenomenon is still largely unknown. One of the possible causes is the diversity of endophytic microbial communities between these two fleshy stems. Therefore, we utilized fungal ITS1 and bacterial 16S rDNA amplicon sequencing to investigate the diversity of endophytic microbial communities in the two different fleshy stems of The results revealed that the α diversity and richness of endophytic fungi in white were significantly greater than in gray . The dominant fungal genus in both fleshy stems was , which accounted for over 90% of the endophytic fungi. The community composition of endophytic fungi in gray and white was different except for , and a negative correlation was observed between and other endophytic fungi. In addition, the dominant bacterial genus in gray was which is also negatively correlated with other bacterium communities. Additionally, the co-occurrence network of white was found to have a stronger scale, connectivity, and complexity compared to that of gray . And the detected beneficial bacteria and pathogens in the stems of potentially compete for resources. Furthermore, the function of endophytic bacteria is more abundant than endophytic fungi in . This research investigated the correlation between the development of fleshy stems and endophytic microbial communities. Our findings indicate that the composition of endophytic microbial communities is closely related to the type of fleshy stems. This research also suggests the potential utilization of specific microbial communities to enhance the growth and development of , thereby contributing to the breeding of .
该植物通常被专性寄生真菌感染，形成可食用的肉质茎，在中国是一种叫做茭白的水生蔬菜。黑粉菌的冬孢子（T）菌株感染会诱导其形成灰色肉质茎，而黑粉菌的菌丝体 - 冬孢子（MT）菌株感染会诱导其形成白色肉质茎，白色肉质茎比灰色肉质茎更适合食用。这种现象的机制在很大程度上仍然未知。一个可能的原因是这两种肉质茎内生微生物群落的多样性。因此，我们利用真菌ITS1和细菌16S rDNA扩增子测序来研究该植物两种不同肉质茎中内生微生物群落的多样性。结果表明，白色肉质茎中内生真菌的α多样性和丰富度显著高于灰色肉质茎。两种肉质茎中优势真菌属均为黑粉菌属，其占内生真菌的比例超过90%。除黑粉菌属外，灰色和白色肉质茎中内生真菌的群落组成不同，并且观察到黑粉菌属与其他内生真菌之间呈负相关。此外，灰色肉质茎中的优势细菌属为假单胞菌属，其也与其他细菌群落呈负相关。另外，发现白色肉质茎的共现网络比灰色肉质茎具有更强的规模、连通性和复杂性。并且在该植物茎中检测到的有益细菌和病原体可能会争夺资源。此外，该植物中内生细菌的功能比内生真菌更丰富。本研究调查了该植物肉质茎发育与内生微生物群落之间的相关性。我们的研究结果表明，内生微生物群落的组成与该植物肉质茎的类型密切相关。本研究还表明了利用特定微生物群落促进该植物生长发育的潜力，从而有助于该植物的育种。
