Zhang Yafen, Hu Yingli, Cao Qianchao, Yin Yumei, Xia Wenqiang, Cui Haifeng, Yu Xiaoping, Ye Zihong
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, China.
Front Microbiol. 2020 Jun 9;11:1053. doi: 10.3389/fmicb.2020.01053. eCollection 2020.
undergoes an endophytic life cycle in . It induces the stem of its host to swell, forming the edible galls called in China, which are the second most commonly cultivated aquatic vegetable in China. raised for can only reproduce asexually because the infection completely inhibits flowering. The infection and proliferation in the host plants during the formation of edible gall differ from those of conventional pathogens. Previous studies have shown a close relationship between mitogen-activated protein kinase (MAPK) and fungal pathogenesis. In this study, we explored the functional properties of the MAPK UeKpp2. Cross-species complementation assays were carried out, which indicated a functional complementation between the of . and the of . Next, mutants of the UeT14 and the UeT55 sporidia background were generated; these showed an aberrant morphology of budding cells, and attenuated mating and filamentous growth , in the context of normal pathogenicity. Interestingly, we identified another protein kinase, UeUkc1, which acted downstream of UeKpp2 and may participate in the regulation of cell shape. We also found a defect of filamentous growth in mutants that was not related to a defect of the induction of mating-type genes but was directly related to a defect in induction. Overall, our results indicate an important role for UeKpp2 in . that is slightly different from those reported for other smut fungi.
在……中经历内生生活周期。它诱导宿主茎部肿胀，在中国形成被称为……的可食用虫瘿，这是中国第二大常见栽培水生蔬菜。用于……种植的……只能进行无性繁殖，因为……感染完全抑制开花。在可食用虫瘿形成过程中，其在宿主植物中的感染和增殖与传统病原体不同。先前的研究表明丝裂原活化蛋白激酶（MAPK）与真菌致病机制密切相关。在本研究中，我们探究了MAPK UeKpp2的功能特性。进行了跨物种互补试验，结果表明……的……与……的……之间存在功能互补。接下来，构建了UeT14和UeT55担孢子背景的突变体；这些突变体在正常致病性的情况下，芽殖细胞呈现异常形态，交配和丝状生长减弱。有趣的是，我们鉴定出另一种蛋白激酶UeUkc1，它在UeKpp2下游起作用，可能参与细胞形态的调控。我们还发现……突变体中丝状生长存在缺陷，这与交配型基因诱导缺陷无关，而是直接与……诱导缺陷有关。总体而言，我们的结果表明UeKpp2在……中发挥重要作用，这与其他黑粉菌报道的情况略有不同。
