Elgegren Mariela, Nakamatsu Javier, Galarreta Betty, Kim Suyeon
Department of Science, Chemistry Division, Pontificia Universidad Catolica del Peru PUCP, Av. Universitaria 1801, Lima 32, Peru.
Department of Engineering, Pontificia Universidad Catolica del Peru PUCP, Av. Universitaria 1801, Lima 32, Peru.
Gels. 2023 Oct 26;9(11):847. doi: 10.3390/gels9110847.
Skin wound healing is a complex biological process of tissue regeneration in which the wound dressing is crucial for rapid healing; it must protect the wound keep an adequate level of moisture and prevent infections. Alginate (AL), a polysaccharide from brown algae, has been extensively studied for wound treatment, and aloe vera gels (AVGs) have also been used in the treatment of skin. The AVG main bioactive polysaccharide was combined with AL for the preparation of membranes. Two-dimensional membranes were prepared by casting and, for comparison, transparent nanoparticle 3D membranes were produced by high-intensity ultrasonication followed by ionotropic crosslinking. The effects of the amount of AVG, ionotropic gelation, and the structure (2D or 3D) of the AL-AVG membranes were compared. Scanning electron microscopy (SEM) showed higher surface roughness on 3D membranes. Three-dimensional membranes showed a higher swelling ratio, and swelling increased with AVG content and decreased with higher calcium concentration and longer gelation times. The degradation of the membranes was evaluated with and without a lysozyme at pH 5.5, 7.5, and 8.5, to simulate different skin conditions; the results evidence that pH had a higher effect than the enzyme. The cytotoxicity of the membranes was evaluated with ATCC CCL 163 and ATCC CCL 81 cells, and an excellent biocompatibility of both cell types (>90% of cell viability after 48 h incubation) was observed for all AL-AVG membranes.
皮肤伤口愈合是一个复杂的组织再生生物学过程，其中伤口敷料对于快速愈合至关重要；它必须保护伤口、保持适当的湿度水平并防止感染。藻酸盐（AL）是一种来自褐藻的多糖，已被广泛研究用于伤口治疗，芦荟凝胶（AVG）也已用于皮肤治疗。将AVG的主要生物活性多糖与AL结合用于制备膜。通过浇铸制备二维膜，作为比较，通过高强度超声处理然后离子交联制备透明纳米颗粒三维膜。比较了AVG用量、离子凝胶化以及AL-AVG膜的结构（二维或三维）的影响。扫描电子显微镜（SEM）显示三维膜的表面粗糙度更高。三维膜显示出更高的溶胀率，溶胀随着AVG含量的增加而增加，随着钙浓度的升高和凝胶化时间的延长而降低。在pH 5.5、7.5和8.5条件下，在有和没有溶菌酶的情况下评估膜的降解情况，以模拟不同的皮肤状况；结果表明pH的影响比酶更大。用ATCC CCL 163和ATCC CCL 81细胞评估膜的细胞毒性，观察到所有AL-AVG膜对两种细胞类型都具有优异的生物相容性（孵育48小时后细胞活力>90%）。
