Koga Adriana Yuriko, Felix Julio César, Silvestre Rodrigo Gomes Marques, Lipinski Leandro Cavalcante, Carletto Bruna, Kawahara Fernanda Alexia, Pereira Airton Vicente
Fellow PhD degree, Postgraduate Program in Pharmaceutical Sciences, Universidade Estadual de Ponta Grossa (UEPG), Brazil. Technical procedures, analysis and interpretation of data, manuscript writing.
Professor, Director in Technology Institute of Paraná, Curitiba-PR, Brazil. Scientific, conception and design of the study.
Acta Cir Bras. 2020 Jul 3;35(5):e202000507. doi: 10.1590/s0102-865020200050000007.
To develop a new wound dressing composed of alginate and Aloe vera gel and cross-linked with zinc ions.
The aloe-alginate film was characterized using scanning electron microscopy (SEM), swelling profile, mechanical properties, polysaccharide content and X-ray diffraction (XRD). Thirty Wistar rats were divided in two groups a) treated with aloe-alginate film and b) control (treated with sterile gauze). Wound contraction measurements and hystological analysis were performed on 7th, 14th and 21st days after wound surgery.
The aloe-alginate film presented adequated mechanical resistance and malleability for application as wound dressing. There was no statistical difference in wound contraction between two groups. Histological assay demonstrated that aloe-alginate film presented anti-inflammatory activity, stimulated angiogenesis on proliferative phase and a more significant increased in collagen type I fibers and decreased type III fibers which promoted a mature scar formation when compared to control.
The aloe-alginate film showed adequate physicochemical characteristics for wound dressing applications. The in vivo assay demonstrated that aloe-alginate film enhanced the healing process of incisional skin wounds.
研发一种由藻酸盐和芦荟凝胶组成并与锌离子交联的新型伤口敷料。
使用扫描电子显微镜（SEM）、溶胀曲线、力学性能、多糖含量和X射线衍射（XRD）对芦荟 - 藻酸盐膜进行表征。将30只Wistar大鼠分为两组：a）用芦荟 - 藻酸盐膜治疗组和b）对照组（用无菌纱布治疗）。在伤口手术后第7天、14天和21天进行伤口收缩测量和组织学分析。
芦荟 - 藻酸盐膜表现出足够的机械抗性和延展性，适用于伤口敷料。两组之间的伤口收缩没有统计学差异。组织学分析表明，与对照组相比，芦荟 - 藻酸盐膜具有抗炎活性，在增殖期刺激血管生成，I型胶原纤维显著增加，III型纤维减少，促进了成熟瘢痕的形成。
芦荟 - 藻酸盐膜显示出适用于伤口敷料应用的足够理化特性。体内试验表明，芦荟 - 藻酸盐膜可促进切口皮肤伤口的愈合过程。
