Yaneva Zvezdelina, Beev Georgi, Rusenova Nikolina, Ivanova Donika, Tzanova Milena, Stoeva Daniela, Toneva Monika
Chemistry Unit, Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria.
Department of Biochemistry, Microbiology and Physics, Faculty of Agriculture, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria.
Antibiotics (Basel). 2022 May 12;11(5):650. doi: 10.3390/antibiotics11050650.
As natural biopolymers, chitosan and lignin are characterized by their good biocompatibility, high biodegradability and satisfactory biosafety. The active polymers’ functional groups are responsible for the potential of these biomaterials for use as carrier matrices in the construction of polymer−drug conjugates with prospective applicability in the fields of medicine, food and agriculture—subjects that have attracted attention in recent years. Hence, the aim of this research was to place substantial emphasis on the antimicrobial potential of flavonoid−biopolymer complex systems by assessment of the probable synergetic, additive or antagonistic effects arising as a function of systemic complexity. The joint implementation of morin, chitosan and lignin in conjugated two- and three-component systems provoked species-dependent antimicrobial synergistic and/or potentiation effects against the activity of the tested bacterial strains Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and the clinical isolate Bacillus cereus. The double combinations of morin−chitosan and morin−lignin resulted in a 100% increase in their inhibitory activity against S. aureus as compared to the pure biocompounds. The inhibitory effects of the three-component system, in decreasing order, were: S. aureus (IZ = 15.7 mm) > P. aeruginosa (IZ = 15 mm) > B. cereus and E. coli (IZ = 14 mm). All tested morin-containing two- and three-component systems exhibited clear and significant potentiation effects, especially against S. aureus and B. cereus. The results obtained are a prerequisite for the potential use of the studied conjugated lignin−morin−chitosan combinations in the construction of novel drug-carrier formulations with improved bioactivities.
作为天然生物聚合物，壳聚糖和木质素具有良好的生物相容性、高生物降解性和令人满意的生物安全性。这些活性聚合物的官能团决定了这些生物材料作为载体基质用于构建聚合物-药物共轭物的潜力，在医学、食品和农业领域具有潜在的应用前景，这些领域近年来备受关注。因此，本研究的目的是通过评估随系统复杂性产生的可能的协同、相加或拮抗作用，着重研究类黄酮-生物聚合物复合体系的抗菌潜力。在共轭的二元和三元体系中联合使用桑色素、壳聚糖和木质素，对测试的金黄色葡萄球菌ATCC 25923、大肠杆菌ATCC 25922、铜绿假单胞菌ATCC 27853和临床分离株蜡样芽孢杆菌产生了物种依赖性的抗菌协同和/或增强作用。与纯生物化合物相比，桑色素-壳聚糖和桑色素-木质素的二元组合对金黄色葡萄球菌的抑制活性提高了100%。三元体系的抑制作用由强到弱依次为：金黄色葡萄球菌（抑菌圈直径IZ = 15.7 mm）＞铜绿假单胞菌（IZ = 15 mm）＞蜡样芽孢杆菌和大肠杆菌（IZ = 14 mm）。所有测试的含桑色素的二元和三元体系均表现出明显且显著的增强作用，尤其是对金黄色葡萄球菌和蜡样芽孢杆菌。所得结果是研究的共轭木质素-桑色素-壳聚糖组合在构建具有改善生物活性的新型药物载体配方中潜在应用的前提条件。
