College of Life Sciences/National Center for Soybean Improvement/Nanjing Agricultural University, Nanjing, 210095, China; Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
College of Life Sciences/National Center for Soybean Improvement/Nanjing Agricultural University, Nanjing, 210095, China; College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, 210095, China.
Plant Physiol Biochem. 2018 Jun;127:25-31. doi: 10.1016/j.plaphy.2018.03.007. Epub 2018 Mar 7.
In plants, various proteins are regulated by the ubiquitin-mediated system in response to different environmental stresses, such as drought, cold and heat. The Skp1-Cullin-F-box (SCF) complex, one of the multisubunit E3 ligases, has been shown to be involved in abiotic response pathways. In this study, Glycine max SKP1-like 1 (GmSK1), which had the typical characteristics of an SKP1 protein, with an alpha/beta structure, targeted to the cytoplasm and nucleus, was isolated from soybean [Glycine max (L.)]. GmSK1 was constitutively expressed in all the tested tissues, especially in the roots. Furthermore, the expression of GmSK1 was simultaneously induced by abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), NaCl, low temperatures and drought, which suggests important roles for GmSK1 in plant responses to hormone treatments and abiotic stress. GmSK1-overexpressing transgenic tobacco (Nicotiana tobacum cv. Samsun) plants showed enhanced tolerance to high salinity and drought stress; exhibited significantly reduced inhibition of growth, greenness and water loss; and exhibited increased MDA accumulation compared with wild-type controls. Our results suggest that GmSK1 might play a role in the crosstalk between ubiquitination and abiotic stress responses in plants.
在植物中，各种蛋白质通过泛素介导的系统来响应不同的环境胁迫（如干旱、寒冷和高温）进行调节。Skp1-Cullin-F-box (SCF) 复合物作为多亚基 E3 连接酶之一，已被证明参与非生物响应途径。本研究从大豆 [Glycine max (L.)] 中分离到 Glycine max SKP1-like 1 (GmSK1)，它具有 SKP1 蛋白的典型特征，具有 α/β 结构，靶向细胞质和细胞核。GmSK1 在所有测试的组织中持续表达，特别是在根部。此外，GmSK1 的表达同时被脱落酸 (ABA)、茉莉酸 (JA)、水杨酸 (SA)、NaCl、低温和干旱诱导，这表明 GmSK1 在植物对激素处理和非生物胁迫的响应中具有重要作用。GmSK1 过表达的转基因烟草 (Nicotiana tobacum cv. Samsun) 植株对高盐和干旱胁迫表现出增强的耐受性；生长、绿色度和水分损失的抑制明显减少；与野生型对照相比，MDA 积累增加。我们的结果表明，GmSK1 可能在植物中泛素化和非生物胁迫响应的串扰中发挥作用。
