Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
Bioengineering Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
Int J Mol Sci. 2023 Nov 20;24(22):16512. doi: 10.3390/ijms242216512.
Much attention has been gained on green silver nanoparticles (green-AgNPs) in the medical field due to their remarkable effects against multi-drug resistant (MDR) microorganisms and targeted cancer treatment. In the current study, we demonstrated a simple and environment-friendly (i.e., green) AgNP synthesis utilizing aqueous leaf extract. This leaf is well-known for its medicinal properties and acts as a reducing and stabilizing agent. Nanoparticle preparation with the desired size and shape was controlled by distinct parameters; for instance, temperature, extract concentration of salt, and pH. The characterization of biosynthesized AgNPs was performed by the UV-spectroscopy technique, dynamic light scattering, scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared. The successful formation of AgNPs was confirmed by a surface plasmon resonance at 422 nm using UV-visible spectroscopy and color change observation with a particle size of 37± 10 nm and a zeta potential of -10.9 ± 2.3 mV. SEM further confirmed the spherical size and shape of AgNPs with a size varying from 28 to 52 nm. Antibacterial activity of the AgNPs was confirmed against all Gram-negative and Gram-positive bacterial reference and MDR strains that were used in different inhibitory rates, and the highest effect was on the reference strain (MIC = 25 μg/mL). The anticancer study of AgNPs exhibited an IC of 1.37 μg/mL and 1.98 μg/mL against MCF-7 (breast cancer cells) and A549 (lung cancer cells), respectively. Therefore, this green synthesis of AgNPs could have a potential clinical application, and further in vivo study is required to assess their safety and efficacy.
由于绿色银纳米粒子（green-AgNPs）在对抗多药耐药（MDR）微生物和靶向癌症治疗方面的显著效果，它们在医学领域引起了广泛关注。在当前的研究中，我们利用水提植物叶片展示了一种简单且环保（即绿色）的 AgNP 合成方法。这种叶子因其药用特性而闻名，可作为还原剂和稳定剂。通过不同的参数，如温度、盐提取物浓度和 pH 值，控制纳米粒子的期望尺寸和形状。通过紫外-可见光谱技术、动态光散射、扫描电子显微镜、X 射线衍射和傅里叶变换红外光谱对生物合成的 AgNPs 进行了表征。成功地通过紫外-可见光谱在 422nm 处的表面等离子体共振和粒径为 37±10nm 和 zeta 电位为-10.9±2.3mV 的粒径观察到颜色变化来确认 AgNPs 的形成。SEM 进一步证实了 AgNPs 的球形尺寸和形状，粒径大小从 28nm 到 52nm 不等。AgNPs 的抗菌活性被证实对所有革兰氏阴性和革兰氏阳性细菌参考菌株和 MDR 菌株均有抑制作用，其抑制率不同，对参考菌株的效果最高（MIC=25μg/mL）。AgNPs 的抗癌研究表明，对 MCF-7（乳腺癌细胞）和 A549（肺癌细胞）的 IC 分别为 1.37μg/mL 和 1.98μg/mL。因此，这种绿色 AgNPs 合成具有潜在的临床应用价值，需要进一步的体内研究来评估其安全性和疗效。
