Li Qiannan, Lyu Chunming, Chen Daqin, Cai Wanling, Kou Fang, Li Qiang, Wei Hai, Zhang Huimin
Department of Dermatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Pharmaceuticals (Basel). 2023 Oct 24;16(11):1514. doi: 10.3390/ph16111514.
Hypertrophic scars (HSs) develop due to excessive collagen deposition and abnormal fibroblast proliferation during wound healing, significantly impacting patient quality of life. Three dosages of GA ointments were administered to rabbit ear HS models to investigate the potential efficacy and mechanism of gallic acid (GA) on HS. Daily application of ointment was performed on the matrix group, the GA ointment groups, and the silicone gel group for 28 days. (No drug treatment was performed on the skin and model groups as a blank group and vehicle group, and silicone gel ointment was topically administered to the silicone gel group as a positive control group.) Scar specimens were collected for histopathology analysis, RNA sequencing analysis, real-time quantitative polymerase chain reaction, and Western blot analysis at the first, second, and fourth weeks after the treatment. Low-dose and medium-dose GA effectively suppressed HS formation and markedly decreased fibroblast infiltration levels and scar thickness. Moreover, decreased expression of TRPC3 mRNA and TGF-β1, p-Smad2/3, and Smad2/3 protein was observed in the low- and medium-dose GA groups and the silicone gel group. This study provides evidence for the efficacy of GA in treating HS and sheds light on its potential underlying pharmacological mechanisms.
增生性瘢痕（HSs）是由于伤口愈合过程中胶原蛋白过度沉积和成纤维细胞异常增殖而形成的，严重影响患者的生活质量。将三种剂量的没食子酸（GA）软膏应用于兔耳HS模型，以研究GA对HS的潜在疗效和作用机制。对基质组、GA软膏组和硅胶凝胶组每日涂抹软膏，持续28天。（皮肤组和模型组不进行药物治疗，分别作为空白组和赋形剂组，硅胶凝胶组局部涂抹硅胶凝胶软膏作为阳性对照组。）在治疗后的第一、第二和第四周收集瘢痕标本进行组织病理学分析、RNA测序分析、实时定量聚合酶链反应和蛋白质印迹分析。低剂量和中剂量GA有效抑制了HS的形成，并显著降低了成纤维细胞浸润水平和瘢痕厚度。此外，在低剂量和中剂量GA组以及硅胶凝胶组中观察到TRPC3 mRNA、TGF-β1、p-Smad2/3和Smad2/3蛋白的表达降低。本研究为GA治疗HS的疗效提供了证据，并揭示了其潜在的药理学机制。
