Gavrila Adina I, Zalaru Christina M, Tatia Rodica, Seciu-Grama Ana-Maria, Negrea Cristina L, Calinescu Ioan, Chipurici Petre, Trifan Adrian, Popa Ioana
Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania.
Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 050663 Bucharest, Romania.
Plants (Basel). 2023 Nov 20;12(22):3908. doi: 10.3390/plants12223908.
L. contains phytochemicals with good biological properties which are beneficial to human health and can be used to protect plants against different diseases. The aim of this research was to find the most suitable extraction method and the most favorable parameters for the extraction of different bioactive compounds from ivy leaves. Different extraction methods, namely microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), and conventional heating extraction (CHE), were used. The most suitable method for the extraction of saponins is MAE with an extraction efficiency of 58%, while for carbohydrates and polyphenols, the best results were achieved via UAE with an extraction efficiency of 61.7% and 63.5%, respectively. The antioxidant activity (AA) of the extracts was also determined. The highest AA was obtained via UAE (368.98 ± 9.01 µmol TR/gDM). Better results were achieved at 50 °C for 10 min of extraction, using 80% ethanol in water as solvent. In order to evaluate their in vitro cytotoxicity, the extracts richest in bioactive compounds were tested on NCTC fibroblasts. Their influence on the DNA content of RAW 264.7 murine macrophages was also tested. Until 200 µg/mL, the extracts obtained via UAE and MAE were cytocompatible with NCTC fibroblasts at 48 h of treatment. Summarizing the above, both MAE and UAE can be employed as green and efficient methods for producing extracts rich in bioactive compounds, exhibiting strong antioxidant properties and good noncytotoxic activity.
常春藤含有具有良好生物学特性的植物化学物质，这些物质对人体健康有益，可用于保护植物免受不同疾病的侵害。本研究的目的是找到从常春藤叶中提取不同生物活性化合物的最合适提取方法和最有利参数。使用了不同的提取方法，即微波辅助提取（MAE）、超声辅助提取（UAE）和传统加热提取（CHE）。提取皂苷最合适的方法是MAE，提取效率为58%，而对于碳水化合物和多酚，通过UAE获得了最佳结果，提取效率分别为61.7%和63.5%。还测定了提取物的抗氧化活性（AA）。通过UAE获得了最高的AA（368.98±9.01μmol TR/gDM）。在50℃下提取10分钟，使用80%乙醇水溶液作为溶剂可获得更好的结果。为了评估它们的体外细胞毒性，对富含生物活性化合物的提取物在NCTC成纤维细胞上进行了测试。还测试了它们对RAW 264.7小鼠巨噬细胞DNA含量的影响。在处理48小时时，直到200μg/mL，通过UAE和MAE获得的提取物与NCTC成纤维细胞具有细胞相容性。综上所述，MAE和UAE都可以作为绿色高效的方法来生产富含生物活性化合物的提取物，这些提取物具有很强的抗氧化特性和良好的非细胞毒性活性。
