Shokry Aya A, El-Shiekh Riham A, Kamel Gehan, Bakr Alaa F, Ramadan Amer
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
Heliyon. 2022 May 19;8(5):e09477. doi: 10.1016/j.heliyon.2022.e09477. eCollection 2022 May.
L. (family Araliaceae) is classified as a conventional plant used as a medicinal product in the cure and prevention of upper respiratory tract inflammation and infection due to its secretolytic and broncholytic effects. Our research was conducted to authenticate the anti-inflammatory effect of ivy leaves extract in the prevention of acute lung injury (ALI) caused by intranasal administration of lipopolysaccharides (LPS). antimicrobial, anti-inflammatory, and anti-oxidant were evaluated, in addition to the acute lung inflammation model induced by LPS in mice. The animals were divided into seven groups randomly (each group containing 10 mice): control negative (saline only), control positive (LPS group), standard (Dexamethasone 2 mg/kg), ethanolic ivy leaves extract (EIE, 100 mg/kg), ethanolic ivy leaves extract (EIE, 200 mg/kg), saponin rich fraction (SRF, 100 mg/kg) and phenolic rich fraction (PRF, 100 mg/kg). Right lungs were homogenized to determine the levels of SOD, MDA, catalase, IL-10, TNF-α, NO, IL-1β, IL-6, PGE2, and MPO. Left lungs were excised for histopathology and histomorphometry. Immunohistochemistry of Cox-2 and TNF-α levels were measured. Additionally, Western blotting was used to determine the levels of phosphorylated MAPK. Also, the ethanolic extract was also standardized through HPLC analysis for its content of rutin.The data showed that the oral supplementation with EIE, 200 mg/kg significantly ( < 0.05) decreased the pro-inflammatory mediators, and oxidative stress biomarkers induced by LPS. Interestingly, the phenolics showed promising activity, therefore they are responsible for the action. In conclusion, the standardized ivy leaf extract could be advised for acute lung injury for its antimicrobial, anti-oxidant, and anti-inflammatory activities.
常春藤（五加科）因其具有溶解分泌物和溶解支气管黏液的作用，被归类为用于治疗和预防上呼吸道炎症及感染的传统药用植物。我们开展此项研究旨在验证常春藤叶提取物在预防因鼻内给予脂多糖（LPS）所致急性肺损伤（ALI）方面的抗炎作用。除了在LPS诱导的小鼠急性肺炎症模型中进行评估外，还对其抗菌、抗炎和抗氧化作用进行了评价。将动物随机分为七组（每组10只小鼠）：阴性对照（仅给予生理盐水）、阳性对照（LPS组）、标准组（地塞米松2mg/kg）、乙醇常春藤叶提取物（EIE，100mg/kg）、乙醇常春藤叶提取物（EIE，200mg/kg）、富含皂苷组分（SRF，100mg/kg）和富含酚类组分（PRF，100mg/kg）。取右肺匀浆以测定超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）、一氧化氮（NO）、白细胞介素-1β（IL-β）、白细胞介素-6（IL-6）、前列腺素E2（PGE2）和髓过氧化物酶（MPO）水平。取左肺用于组织病理学和组织形态计量学分析。测定环氧化酶-2（Cox-2）和TNF-α水平的免疫组织化学分析。此外，采用蛋白质免疫印迹法测定磷酸化丝裂原活化蛋白激酶（MAPK）水平。同时，通过高效液相色谱（HPLC）分析对乙醇提取物中的芦丁含量进行标准化测定。数据显示，口服给予200mg/kg的EIE可显著（P<0.05）降低LPS诱导的促炎介质和氧化应激生物标志物水平。有趣的是，酚类物质显示出良好的活性，因此它们是发挥作用的原因。总之，鉴于其抗菌、抗氧化和抗炎活性，标准化的常春藤叶提取物可用于急性肺损伤。
