使用抗生素消除蚊子和细胞培养物中的沃尔巴克氏体。

Use of Antibiotics to Eliminate Wolbachia from Mosquitoes and Cell Culture.

机构信息

Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, Australia.

QIMR Berghofer Medical Research Institute, Herston, Australia.

出版信息

Methods Mol Biol. 2024;2739:147-156. doi: 10.1007/978-1-0716-3553-7_9.

DOI:10.1007/978-1-0716-3553-7_9

PMID:38006550

Abstract

Removal of Wolbachia from infected insects is required in most experimental settings when the effects of Wolbachia on biological traits, pathogen blocking, reproduction, and fitness are assessed. This is to ensure that the genetic backgrounds of Wolbachia-infected and uninfected insects are the same. Here, we describe methodologies used for the elimination of Wolbachia from insects and insect cell lines with antibiotics.

摘要

在评估沃尔巴克氏体对生物特征、病原体阻断、繁殖和适应性的影响的大多数实验环境中，需要从感染昆虫中去除沃尔巴克氏体。这是为了确保感染和未感染沃尔巴克氏体的昆虫的遗传背景相同。本文描述了使用抗生素从昆虫和昆虫细胞系中消除沃尔巴克氏体的方法。