Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road 501, Shanghai 201203, China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China.
Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road 501, Shanghai 201203, China.
Phytomedicine. 2024 Jan;123:155228. doi: 10.1016/j.phymed.2023.155228. Epub 2023 Nov 19.
Fritillaria Bulbus (FB), a precious medicinal herb renowned for its heat-clearing, lung-moistening, cough-relieving and phlegm-eliminating effects. In pursuit of profits, unscrupulous merchants have engaged in the substitution or adulteration of valuable varieties with cheaper alternatives. It is, therefore, urgent to develop effective technical approaches to identify FBs from adulterants.
This paper employed infrared spectroscopy (IR), thin layer chromatography-image analysis (TLC-IA), and untargeted metabolomics techniques to discriminate ten species of FBs.
Five species of FBs were successfully differentiated using mid-infrared spectroscopy. Furthermore, the power of TLC-IA technology allowed the differentiation of five species of FBs and two origins of FCBs (Fritillariae Cirrhosae Bulbus). Remarkably, through the application of untargeted metabolomics technique, the precise discrimination of five species of FBs, as well as three origins of FCBs were accomplished. Moreover, a comprehensive identification of 101 markers that reliably distinguished diverse FBs was achieved through the employment of untargeted metabolomics technique.
The investigation presented powerful means of detection for assuring the quality control of Fritillaria herbs.
贝母（FB）是一种珍贵的药材，具有清热润肺、止咳化痰的功效。为了追求利润，不法商贩将有价值的品种与更便宜的品种进行替代或掺假。因此，迫切需要开发有效的技术方法来鉴别 FB 及其掺杂物。
本文采用红外光谱（IR）、薄层色谱图像分析（TLC-IA）和非靶向代谢组学技术对十种 FB 进行鉴别。
中红外光谱成功区分了五种 FB。此外，TLC-IA 技术的强大功能可区分五种 FB 和两种 FCb（川贝母）的来源。值得注意的是，通过应用非靶向代谢组学技术，可精确区分五种 FB 和三种 FCb 的来源。此外，非靶向代谢组学技术还可全面鉴定 101 个可靠区分不同 FB 的标记物。
本研究为确保贝母药材的质量控制提供了强有力的检测手段。
