Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China.
Commun Biol. 2023 Nov 25;6(1):1202. doi: 10.1038/s42003-023-05583-7.
Adding adipose cells to cell-cultured meat can provide a distinctive aroma and juicy texture similar to real meat. However, a significant challenge still exists in obtaining seed cells that can be propagated for long periods, maintain their adipogenic potential, and reduce production costs. In this study, we present a cell strain derived from immortalized porcine preadipocytes that can be subculture for over 40 passages without losing differentiation capacity. This cell strain can be differentiated within 3D bioscaffolds to generate cell-cultured fat using fewer chemicals and less serum. Additionally, it can be expanded and differentiated on microcarriers with upscaled culture to reduce costs and labor. Moreover, it can co-differentiate with muscle precursor cells, producing a pattern similar to real meat. Therefore, our cell strain provides an exceptional model for studying and producing cell-cultured fat.
向细胞培养肉中添加脂肪细胞可以提供独特的香气和多汁的质地，类似于真正的肉。然而，仍然存在一个重大挑战，即获得可以长期繁殖、保持其脂肪生成潜力并降低生产成本的种子细胞。在这项研究中，我们提出了一种源自永生化猪前体脂肪细胞的细胞株，该细胞株可以进行超过 40 代的传代培养，而不会失去分化能力。这种细胞株可以在 3D 生物支架中分化，使用更少的化学物质和更少的血清来生成细胞培养脂肪。此外，它可以在微载体上进行扩增和分化，以进行规模化培养，从而降低成本和劳动力。此外，它还可以与肌肉前体细胞共同分化，产生类似于真正肉的模式。因此，我们的细胞株为研究和生产细胞培养脂肪提供了一个极好的模型。
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