Bluett M K, Abumrad N N, Arab N, Ghishan F K
Biochem J. 1986 Jul 1;237(1):229-34. doi: 10.1042/bj2370229.
D-Glucose transport was investigated in isolated brush-border membrane vesicles from human small intestine. Characteristics of D-glucose transport from the jejunum were compared with that in the mid and terminal ileum. Jejunal and mid-ileal D-glucose transport was Na+-dependent and electrogenic. The transient overshoot of jejunal D-glucose transport was significantly greater than corresponding values in mid-ileum. The terminal ileum did not exhibit Na+-dependent D-glucose transport, but did exhibit Na+-dependent taurocholate transport. Na+-glucose co-transport activity as measured by tracer-exchange experiments was greatest in the jejunum, and diminished aborally. We conclude that D-glucose transport in man is Na+-dependent and electrogenic in the proximal intestine and directly related to the activity of D-glucose-Na+ transporters present in the brush-border membranes. D-Glucose transport in the terminal ileum resembles colonic transport of D-glucose.
在从人小肠分离出的刷状缘膜囊泡中研究了D-葡萄糖转运。将空肠中D-葡萄糖转运的特征与回肠中部和末端的特征进行了比较。空肠和回肠中部的D-葡萄糖转运是Na⁺依赖性的且是电生性的。空肠D-葡萄糖转运的瞬时过冲明显大于回肠中部的相应值。回肠末端未表现出Na⁺依赖性D-葡萄糖转运，但表现出Na⁺依赖性牛磺胆酸盐转运。通过示踪剂交换实验测量的Na⁺-葡萄糖共转运活性在空肠中最大，并向口端递减。我们得出结论，人类近端肠道中的D-葡萄糖转运是Na⁺依赖性的且是电生性的，并且与刷状缘膜中存在的D-葡萄糖-Na⁺转运体的活性直接相关。回肠末端的D-葡萄糖转运类似于结肠中D-葡萄糖的转运。
