Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Xiamen, 361005, China.
Anal Bioanal Chem. 2024 Apr;416(9):2203-2220. doi: 10.1007/s00216-023-05028-4. Epub 2023 Nov 26.
Single-cell multiomics technologies empower simultaneous measurement of multiple types of molecules within individual cells, providing a more profound comprehension compared with the analysis of discrete molecular layers from different cells. Microfluidic technology, on the other hand, has emerged as a pivotal facilitator for high-throughput single-cell analysis, offering precise control and manipulation of individual cells. The primary focus of this review encompasses an appraisal of cutting-edge microfluidic platforms employed in the realm of single-cell multiomics analysis. Furthermore, it discusses technological advancements in various single-cell omics such as genomics, transcriptomics, epigenomics, and proteomics, with their perspective applications. Finally, it provides future prospects of these integrated single-cell multiomics methodologies, shedding light on the possibilities for future biological research.
单细胞多组学技术能够在单个细胞内同时测量多种类型的分子，与分析来自不同细胞的离散分子层相比，能够提供更深入的理解。另一方面，微流控技术已成为高通量单细胞分析的关键促进因素，能够精确控制和操作单个细胞。本综述的主要重点是评估单细胞多组学分析领域中使用的最新微流控平台。此外，它还讨论了各种单细胞组学（如基因组学、转录组学、表观基因组学和蛋白质组学）的技术进展，及其潜在的应用。最后，它提供了这些集成的单细胞多组学方法的未来前景，揭示了未来生物研究的可能性。
