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分散的山羊乳腺上皮细胞合成乳特异性脂肪酸和蛋白质。

Synthesis of milk specific fatty acids and proteins by dispersed goat mammary-gland epithelial cells.

作者信息

Hansen H O, Tornehave D, Knudsen J

出版信息

Biochem J. 1986 Aug 15;238(1):167-72. doi: 10.1042/bj2380167.

DOI:10.1042/bj2380167
PMID:3800930
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1147111/
Abstract

The method now described for preparation of dispersed lactating goat mammary-gland cells gives a high yield of morphologically and functionally normal mammary cells. The cells synthesize specific goat milk fatty acids in the right proportions, and they respond to hormones by increased protein synthesis. The cells can be frozen and thawed without losing the above properties, which makes them an excellent tool for metabolic and hormonal studies.

摘要

现在所描述的制备分散的泌乳山羊乳腺细胞的方法,能产生大量形态和功能正常的乳腺细胞。这些细胞能以正确的比例合成特定的山羊奶脂肪酸,并且它们对激素作出反应,蛋白质合成增加。这些细胞可以冷冻和解冻而不丧失上述特性,这使其成为代谢和激素研究的极佳工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5229/1147111/344df82c3f0c/biochemj00273-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5229/1147111/dec3f46f3c34/biochemj00273-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5229/1147111/344df82c3f0c/biochemj00273-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5229/1147111/dec3f46f3c34/biochemj00273-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5229/1147111/344df82c3f0c/biochemj00273-0165-a.jpg

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本文引用的文献

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Body size and tissue respiration.身体大小与组织呼吸
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山羊乳腺中三酰甘油合成的单酰甘油途径缺失。
Biochem J. 1986 Aug 15;238(1):173-6. doi: 10.1042/bj2380173.
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Triacylglycerol synthesis in goat mammary gland. Factors influencing the esterification of fatty acids synthesized de novo.山羊乳腺中的三酰甘油合成。影响从头合成脂肪酸酯化的因素。
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Comparative biochemical studies of milks. IV. Constituent fatty acids of milk fats.牛奶的比较生化研究。IV. 乳脂肪中的组成脂肪酸。
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Biosynthesis of secretory lipids from [2-14C]acetate by bovine mammary cells in vitro.牛乳腺细胞在体外利用[2-¹⁴C]乙酸盐进行分泌性脂质的生物合成。
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Stearic acid metabolism by mammary cells.乳腺细胞的硬脂酸代谢
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The absorption of fatty acids by functional bovine mammary cells.功能性牛乳腺细胞对脂肪酸的吸收。
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Comparative maintenance of function in dispersed cell and organ cultures of bovine mammary tissue.牛乳腺组织分散细胞和器官培养中功能的比较维持
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