Hansen H O, Tornehave D, Knudsen J
Biochem J. 1986 Aug 15;238(1):167-72. doi: 10.1042/bj2380167.
The method now described for preparation of dispersed lactating goat mammary-gland cells gives a high yield of morphologically and functionally normal mammary cells. The cells synthesize specific goat milk fatty acids in the right proportions, and they respond to hormones by increased protein synthesis. The cells can be frozen and thawed without losing the above properties, which makes them an excellent tool for metabolic and hormonal studies.
现在所描述的制备分散的泌乳山羊乳腺细胞的方法,能产生大量形态和功能正常的乳腺细胞。这些细胞能以正确的比例合成特定的山羊奶脂肪酸,并且它们对激素作出反应,蛋白质合成增加。这些细胞可以冷冻和解冻而不丧失上述特性,这使其成为代谢和激素研究的极佳工具。