大鼠静息状态及氰化物中毒后骨骼肌中NADH的含量。
The content of NADH in rat skeletal muscle at rest and after cyanide poisoning.
作者信息
Sahlin K, Katz A
出版信息
Biochem J. 1986 Oct 1;239(1):245-8. doi: 10.1042/bj2390245.
The concentration of NADH was determined a high-oxidative muscle (soleus) and a high-glycolytic muscle (extensor digitorum longus, EDL) from resting rats. The NADH content of freeze-clamped control muscles was 0.35 +/- 0.04 (mean +/- S.D.) and 0.31 +/- 0.04 mmol/kg dry wt. in EDL and soleus respectively, and increased to peak values of 0.58 +/- 0.05 (EDL) and 0.87 +/- 0.10 (soleus) after 10 min of NaCN treatment. The [lactate]/[pyruvate] ratio, which was not significantly changed in soleus and increased only slightly in EDL after NaCN incubation, shows that only minor changes occurred in the cytosolic NADH concentration. Provided that the major part of muscle NADH is located in the mitochondria it can be calculated that the mitochondrial NADH content in skeletal muscle at rest is about 36 (soleus) and 60% (EDL) of the anoxic value, respectively. These results are in contrast with previous studies with the surface-fluorescence technique, where mitochondrial NAD appeared to be almost completely reduced in resting skeletal muscle.
测定了静息大鼠高氧化型肌肉（比目鱼肌）和高糖酵解型肌肉（趾长伸肌，EDL）中NADH的浓度。冷冻钳夹的对照肌肉中，EDL和比目鱼肌的NADH含量分别为0.35±0.04（平均值±标准差）和0.31±0.04 mmol/kg干重，在NaCN处理10分钟后分别增加到峰值0.58±0.05（EDL）和0.87±0.10（比目鱼肌）。[乳酸]/[丙酮酸]比值在比目鱼肌中无显著变化，在NaCN孵育后EDL中仅略有增加，这表明胞质NADH浓度仅发生了微小变化。假设肌肉NADH的主要部分位于线粒体中，可以计算出静息骨骼肌中线粒体NADH含量分别约为缺氧值的36%（比目鱼肌）和60%（EDL）。这些结果与之前使用表面荧光技术的研究结果相反，在之前的研究中，静息骨骼肌中的线粒体NAD似乎几乎完全被还原。
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