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单细胞 RNA 测序揭示小细胞肺癌相关恶性胸腔积液的免疫微环境。

Single-cell RNA sequencing reveals immune microenvironment of small cell lung cancer-associated malignant pleural effusion.

机构信息

Department of Respiratory and Critical Care Medicine, West China Hospital of Sichuan University, Chengdu, China.

Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, China.

出版信息

Thorac Cancer. 2024 Jan;15(1):98-103. doi: 10.1111/1759-7714.15145. Epub 2023 Nov 27.

DOI:10.1111/1759-7714.15145
PMID:38010064
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10761622/
Abstract

We used 10 × genomics single-cell transcriptome sequencing technology to reveal the tumor immune microenvironment characteristics of small cell lung cancer (SCLC) in a patient with malignant pleural effusion (MPE). A total of 8008 high-quality cells were finally obtained for subsequent bioinformatic analysis, which were divided into 10 cell clusters further identified as B cells, T cells, myeloid cells, NK cells, and cancer cells. Such SCLC related genes as NOTCH1, MYC, TSC22D1, SOX4, BLNK, YBX3, VIM, CD8A, CD8B, and KLF6 were expressed in different degrees during differentiation of T and B cells. Different ligands and receptors between T, B and tumor cells almost interact through MHC II, IL-16, galectin, and APP signaling pathway.

摘要

我们使用 10× 基因组单细胞转录组测序技术揭示了恶性胸腔积液（MPE）患者小细胞肺癌（SCLC）的肿瘤免疫微环境特征。最终共获得了 8008 个高质量细胞，用于后续的生物信息学分析，这些细胞被进一步分为 10 个细胞簇，进一步鉴定为 B 细胞、T 细胞、髓样细胞、NK 细胞和癌细胞。在 T 和 B 细胞分化过程中，NOTCH1、MYC、TSC22D1、SOX4、BLNK、YBX3、VIM、CD8A、CD8B 和 KLF6 等 SCLC 相关基因表达程度不同。T、B 和肿瘤细胞之间的不同配体和受体几乎通过 MHC II、IL-16、半乳糖凝集素和 APP 信号通路相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/5cc47161decc/TCA-15-98-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/7378d905fa6b/TCA-15-98-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/944d6c5504b4/TCA-15-98-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/2c68105552f9/TCA-15-98-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/251c52c609cf/TCA-15-98-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/5cc47161decc/TCA-15-98-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/7378d905fa6b/TCA-15-98-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/944d6c5504b4/TCA-15-98-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/2c68105552f9/TCA-15-98-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/251c52c609cf/TCA-15-98-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1777/10761622/5cc47161decc/TCA-15-98-g004.jpg

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2
Understanding SCLC heterogeneity and plasticity in cancer metastasis and chemotherapy resistance.解析小细胞肺癌异质性和可塑性在癌症转移和化疗耐药中的作用。
Acta Biochim Biophys Sin (Shanghai). 2023 May 30;55(6):948-955. doi: 10.3724/abbs.2023080.
3
SCLC Treatment in the Immuno-Oncology Era: Current Evidence and Unmet Needs.
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Front Oncol. 2022 Apr 14;12:840783. doi: 10.3389/fonc.2022.840783. eCollection 2022.
4
Small cell lung cancer: Subtypes and therapeutic implications.小细胞肺癌：亚型与治疗意义。
Semin Cancer Biol. 2022 Nov;86(Pt 2):543-554. doi: 10.1016/j.semcancer.2022.04.001. Epub 2022 Apr 6.
5
A phase II study of anlotinib combined with etoposide and platinum-based regimens in the first-line treatment of extensive-stage small cell lung cancer.一项安罗替尼联合依托泊苷和铂类方案一线治疗广泛期小细胞肺癌的 II 期研究。
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6
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7
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