Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
J Biophotonics. 2024 Mar;17(3):e202300384. doi: 10.1002/jbio.202300384. Epub 2023 Dec 15.
Pseudomonas aeruginosa (P. aeruginosa) is a multidrug-resistant human pathogen involved in numerous infections. Understanding the response of P. aeruginosa to various treatments is critical to developing new ways for the antimicrobial susceptibly test and more effective treatment methods. Conventional antimicrobial susceptibility tests lack molecular information at the single bacterium level. In this study, we used label-free multimodal nonlinear optical microscopy to identify an autofluorescence signal from pyoverdine, a siderophore of the bacteria, for quantification of P. aeruginosa responses to antibiotics and blue light treatment. We also discovered that the bleaching of the pyoverdine autofluorescence signals is correlated with the inactivation of P. aeruginosa and is perhaps one of the mechanisms involved in the blue light inactivation of P. aeruginosa.
铜绿假单胞菌（P. aeruginosa）是一种具有多重耐药性的人类病原体，涉及多种感染。了解铜绿假单胞菌对各种治疗方法的反应对于开发新的抗菌药敏试验方法和更有效的治疗方法至关重要。传统的抗菌药敏试验缺乏单个细菌水平的分子信息。在这项研究中，我们使用无标记多模态非线性光学显微镜来识别来自细菌铁载体的绿脓菌素的自发荧光信号，以定量测定铜绿假单胞菌对抗生素和蓝光处理的反应。我们还发现，绿脓菌素自发荧光信号的漂白与铜绿假单胞菌的失活相关，并且可能是蓝光灭活铜绿假单胞菌的机制之一。
