Department of Electronics and Computer Science, and Institute for Life Science, University of Southampton, Hampshire, SO17 1BJ, UK.
Department of Microbiology, PathWest Laboratory Medicine, Nedlands, WA, 6009, Australia.
Nat Commun. 2020 Oct 21;11(1):5328. doi: 10.1038/s41467-020-18902-x.
There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 10 cells are analysed one-by-one with microfluidic impedance cytometry for 2-3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.
目前迫切需要开发简单、快速的抗菌药物敏感性测试（AST）方法，以实现抗生素的合理使用。在这里，我们描述了一种无需标记的 AST 方法，该方法可以在一小时内提供结果，起始材料为处于活跃分裂状态的培养物。将细菌在抗生素存在的情况下孵育 30 分钟，然后使用微流控阻抗细胞术在 2-3 分钟内逐个分析大约 10 个细胞。所测量的电学特性反映了细菌在 30 分钟孵育窗口内对特定抗生素作用模式的表型反应。该方法的结果与一系列抗生素和细菌物种的经典肉汤微量稀释测定结果一致。
