Gladyshev N S, Baram D V, Gorbunova A V, Krivolapov Yu A
Saint-Petersburg Pasteur Institute, St. Petersburg, Russia.
Russian Scientific Research Institute of Hematology and Transfusiology, St. Petersburg, Russia.
Arkh Patol. 2023;85(6):26-30. doi: 10.17116/patol20238506126.
Metagenomic studies in recent years have demonstrated that all tissues of the human body studied by genomic and transcriptomic sequencing methods, both in pathological processes and in normality, contain fragments of DNA and RNA from a variety of microorganisms. The composition of tissue microbiota and its relationship with development of pathological changes are still poorly understood, despite increasing number of studies in this area every year. In this study, gene expression of the lymph node microbiome in reactive follicular hyperplasia and follicular lymphoma was investigated.
To study expression of lymph node microbiome genes in reactive follicular hyperplasia and follicular lymphoma.
The work included 38 biopsy samples of lymph nodes with follicular lymphoma of different cytological subtypes and 10 biopsy samples of lymph nodes with reactive follicular hyperplasia. Verification of diagnosis was carried out using standard histological, histochemical and immunohistochemical methods. Using sequencing method, the transcriptome was examined. Statistical analysis and data visualization were performed using the R programming language (version 4.2.1).
Tumor lymph nodes are characterized by large Simpson and Shannon alpha diversity values (p-value = 0.026465 and p-value = 0.007122, respectively). Two clusters were discovered, characterized by different levels of relative abundance of microorganisms.
It has been proven that diversity of microorganisms present in tumor tissue and their number are statistically significantly higher than corresponding indicators in the lymph nodes with follicular hyperplasia.
近年来的宏基因组学研究表明，通过基因组和转录组测序方法研究的人体所有组织，无论是在病理过程还是正常状态下，都含有来自多种微生物的DNA和RNA片段。尽管该领域的研究每年都在增加，但组织微生物群的组成及其与病理变化发展的关系仍知之甚少。在本研究中，对反应性滤泡增生和滤泡性淋巴瘤中淋巴结微生物组的基因表达进行了研究。
研究反应性滤泡增生和滤泡性淋巴瘤中淋巴结微生物组基因的表达。
该研究纳入了38例不同细胞学亚型滤泡性淋巴瘤的淋巴结活检样本和10例反应性滤泡增生的淋巴结活检样本。使用标准的组织学、组织化学和免疫组织化学方法进行诊断验证。采用测序方法检测转录组。使用R编程语言（版本4.2.1）进行统计分析和数据可视化。
肿瘤淋巴结的特征是具有较大的辛普森和香农α多样性值（p值分别为0.026465和0.007122）。发现了两个簇，其特征是微生物相对丰度水平不同。
已证实肿瘤组织中存在的微生物多样性及其数量在统计学上显著高于滤泡增生淋巴结中的相应指标。
