Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia, Chile.
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Sevilla, Spain.
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.005983.
As part of a larger study on carried by wild birds in the city of Valdivia (southern Chile), two curved rod-shaped Gram-stain-negative strains (A82 and WB-40) were recovered from faecal samples and subjected to a taxonomic study. Results of a genus-specific PCR showed that these isolates belonged to the genus . Further identification by 16S rRNA and (60 kDa heat-shock protein) gene sequence analysis revealed that they formed a separate phylogenetic clade, different from other known species with '' CNRCH 2005/566H and WBE14 being the most closely related species. This was confirmed by core-genome phylogeny as well as digital DNA-DNA hybridization and average nucleotide identity analyses between the genomes of strains A82 and WB-40 and all other species. The draft genome sequences of A82 and WB-40, obtained by Illumina NextSeq 2000 sequencing, consisted of 1.6 Mb with a G+C content of 31.9-32.0 mol%. The results obtained from the phylogenetic and genomic characterization, together with their different morphological and biochemical features, revealed that these two strains represent a novel species, for which we propose the name sp. nov. with A82 (=LMG 32718=CCCT 22.04) as the type strain.
作为在智利南部城市瓦尔迪维亚(Valdivia)的野生鸟类中进行的一项更大规模研究的一部分,从粪便样本中回收了两株弯曲的杆状革兰氏阴性菌株(A82 和 WB-40),并对其进行了分类学研究。属特异性 PCR 的结果表明,这些分离株属于属 。通过 16S rRNA 和 hsp60 基因序列分析进一步鉴定表明,它们形成了一个单独的系统发育分支,与其他已知的 物种不同,与 '' CNRCH 2005/566H 和 WBE14 最为密切相关。这一点通过核心基因组系统发育以及 A82 和 WB-40 菌株与所有其他 物种之间的基因组 DNA-DNA 杂交和平均核苷酸同一性分析得到了证实。通过 Illumina NextSeq 2000 测序获得的 A82 和 WB-40 的草图基因组序列由 1.6 Mb 组成,GC 含量为 31.9-32.0 mol%。基于系统发育和基因组特征的结果,以及它们不同的形态和生化特征,表明这两株代表了一个新的物种,我们提议将其命名为 sp. nov.,其中 A82(=LMG 32718=CCCT 22.04)为模式株。