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sp. nov.,从黑脸琵鹭()的粪便中分离得到。

sp. nov., isolated from faecal droppings of black-faced ibis ().

机构信息

Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia, Chile.

Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Sevilla, Spain.

出版信息

Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.005983.

Abstract

As part of a larger study on carried by wild birds in the city of Valdivia (southern Chile), two curved rod-shaped Gram-stain-negative strains (A82 and WB-40) were recovered from faecal samples and subjected to a taxonomic study. Results of a genus-specific PCR showed that these isolates belonged to the genus . Further identification by 16S rRNA and (60 kDa heat-shock protein) gene sequence analysis revealed that they formed a separate phylogenetic clade, different from other known species with '' CNRCH 2005/566H and WBE14 being the most closely related species. This was confirmed by core-genome phylogeny as well as digital DNA-DNA hybridization and average nucleotide identity analyses between the genomes of strains A82 and WB-40 and all other species. The draft genome sequences of A82 and WB-40, obtained by Illumina NextSeq 2000 sequencing, consisted of 1.6 Mb with a G+C content of 31.9-32.0 mol%. The results obtained from the phylogenetic and genomic characterization, together with their different morphological and biochemical features, revealed that these two strains represent a novel species, for which we propose the name sp. nov. with A82 (=LMG 32718=CCCT 22.04) as the type strain.

摘要

作为在智利南部城市瓦尔迪维亚(Valdivia)的野生鸟类中进行的一项更大规模研究的一部分,从粪便样本中回收了两株弯曲的杆状革兰氏阴性菌株(A82 和 WB-40),并对其进行了分类学研究。属特异性 PCR 的结果表明,这些分离株属于属 。通过 16S rRNA 和 hsp60 基因序列分析进一步鉴定表明,它们形成了一个单独的系统发育分支,与其他已知的 物种不同,与 '' CNRCH 2005/566H 和 WBE14 最为密切相关。这一点通过核心基因组系统发育以及 A82 和 WB-40 菌株与所有其他 物种之间的基因组 DNA-DNA 杂交和平均核苷酸同一性分析得到了证实。通过 Illumina NextSeq 2000 测序获得的 A82 和 WB-40 的草图基因组序列由 1.6 Mb 组成,GC 含量为 31.9-32.0 mol%。基于系统发育和基因组特征的结果,以及它们不同的形态和生化特征,表明这两株代表了一个新的物种,我们提议将其命名为 sp. nov.,其中 A82(=LMG 32718=CCCT 22.04)为模式株。

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