Department of Microbiology, Faculty of Veterinary Medicine, Erciyes University, 38280 Kayseri, Turkey.
Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Erciyes University, 38280 Kayseri, Turkey.
Syst Appl Microbiol. 2022 Jul;45(4):126326. doi: 10.1016/j.syapm.2022.126326. Epub 2022 May 2.
A total of 26 Gram-negative, motile, gently curved, and rod-shaped isolates were recovered, during a study to determine the faeco-prevalence of Helicobacter spp. in urban wild birds. Pairwise comparisons of the 16S rRNA gene sequences indicated that these isolates belonged to the genus Helicobacter and phylogenetic analysis based on the 16S rRNA gene sequences showed that the isolates were separated into two divergent groups. The first group consisted of 20 urease-positive isolates sharing the highest 16S rRNA gene sequence identity levels of 98.5-98.6% to H. mustelae ATCC 43772, while the second group contained six urease-negative isolates with the sequence identity level of 98.5% to the type strain of H. pametensis ATCC 51478. Five isolates were chosen and subjected to comparative whole-genome analysis. The phylogenetic analysis of the 16S rRNA, gyrA and atpA gene sequences showed that Helicobacter isolates formed two separate phylogenetic clades, differentiating the isolates from the other Helicobacter species. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses between strains faydin-H8, faydin-H23 and their close neighbors H. anseris MIT 04-9362 and H. pametensis ATCC 51478, respectively, confirmed that both strains represent novel species in the genus Helicobacter. The DNA G+C contents of the strains faydin-H8 and faydin-H23 are 32.0% and 37.6%, respectively. The results obtained for the characterization of the wild bird isolates indicate that they represent two novel species, for which the names Helicobacter anatolicus sp. nov., and Helicobacter kayseriensis sp. nov., are proposed, with faydin-H8(=LMG 32237 = DSM 112312) and faydin-H23(=LMG 32236 = CECT 30508) as respective type strains.
在一项研究中，共回收了 26 株革兰氏阴性、运动、轻度弯曲的杆状分离株，用于确定城市野生鸟类中幽门螺杆菌属的粪便流行率。16S rRNA 基因序列的成对比较表明，这些分离株属于幽门螺杆菌属，基于 16S rRNA 基因序列的系统发育分析表明，分离株分为两个不同的群。第一组由 20 株尿素酶阳性分离株组成，与 H. mustelae ATCC 43772 的 16S rRNA 基因序列同源性最高，为 98.5-98.6%，而第二组包含 6 株尿素酶阴性分离株，与 H. pametensis ATCC 51478 的 16S rRNA 基因序列同源性为 98.5%。选择了 5 株分离株进行比较全基因组分析。16S rRNA、gyrA 和 atpA 基因序列的系统发育分析表明，幽门螺杆菌分离株形成了两个独立的系统发育分支，将分离株与其他幽门螺杆菌种区分开来。菌株 faydin-H8、faydin-H23 及其近邻 H. anseris MIT 04-9362 和 H. pametensis ATCC 51478 之间的数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）分析证实，这两个菌株分别代表了幽门螺杆菌属中的两个新种。菌株 faydin-H8 和 faydin-H23 的 DNA G+C 含量分别为 32.0%和 37.6%。对野生鸟类分离株进行的特征描述表明，它们代表了两个新种，分别提议命名为 Helicobacter anatolicus sp. nov. 和 Helicobacter kayseriensis sp. nov.，faydin-H8（=LMG 32237 = DSM 112312）和 faydin-H23（=LMG 32236 = CECT 30508）分别为它们的模式菌株。
