Wang Hongji, Yao Lan, Chen Jie, Ding Zeran, Ou Xuan, Zhang Chaowen, Zhao Jianjun, Han Yuzhu
College of Animal Science and Technology, Southwest University, Chongqing 402460, China.
Immunology Research Center, Institute of Medicine, Southwest University, Chongqing 402460, China.
J Agric Food Chem. 2023 Dec 13;71(49):19638-19651. doi: 10.1021/acs.jafc.3c07953. Epub 2023 Nov 28.
causes wilt disease, which causes huge economic losses to a wide range of agricultural cash crops. Antifungal peptide P852 is an effective biocide. However, the mechanism of direct inhibition of pathogenic fungus needs to be explored. The proteomics and transcriptomics results showed that P852 mainly affected intracellular pathways such as glucose metabolism, amino acid metabolism, and oxidoreductase activity in . P852 disrupts the intracellular oxidative equilibrium in , and transmission electron microscopy observed mitochondrial swelling, disruption of membrane structure, and leakage of contents. Decreased mitochondrial membrane potential, mitochondrial cytochrome leakage, and reduced ATP production were also detected. These results suggest that P852 is able to simultaneously inhibit intracellular metabolism and disrupt the mitochondrial function of , exerting its inhibitory effects in multiple pathways together. The present study provides some insights into the multitargeted mechanism of fungus inhibition of antifungal lipopeptide substances produced by spp.
引起枯萎病，给多种农业经济作物造成巨大经济损失。抗真菌肽P852是一种有效的杀菌剂。然而，其直接抑制病原真菌的机制尚待探索。蛋白质组学和转录组学结果表明，P852主要影响[具体对象]中的细胞内途径，如葡萄糖代谢、氨基酸代谢和氧化还原酶活性。P852破坏了[具体对象]中的细胞内氧化平衡，透射电子显微镜观察到线粒体肿胀、膜结构破坏和内容物泄漏。还检测到线粒体膜电位降低、线粒体细胞色素泄漏和ATP产生减少。这些结果表明，P852能够同时抑制[具体对象]的细胞内代谢并破坏其线粒体功能，通过多种途径共同发挥抑制作用。本研究为[具体菌种]产生的抗真菌脂肽物质抑制真菌的多靶点机制提供了一些见解。
