Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China.
National Key Laboratory of Agricultural Microbiology & Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430000, China.
J Agric Food Chem. 2024 Apr 10;72(14):7943-7953. doi: 10.1021/acs.jafc.4c00126. Epub 2024 Mar 26.
wilt is a worldwide soil-borne fungal disease caused by that causes serious damage to agricultural products. Therefore, preventing and treating fusarium wilt is of great significance. In this study, we purified ten single lipopeptide fengycin components from FAJT-4 and found that C fengycin B inhibited the growth of FJAT-31362. We observed early apoptosis hallmarks, including reactive oxygen species accumulation, mitochondrial dysfunction, and phosphatidylserine externalization in C fengycin B-treated cells. Further data showed that C fengycin B induces cell apoptosis in a metacaspase-dependent manner. Importantly, we found that the expression of autophagy-related genes in the TOR signaling pathway was significantly upregulated; simultaneously, the accumulation of acidic autophagy vacuoles in cell indicated that the autophagy pathway was activated during apoptosis induced by C fengycin B. Therefore, this study provides new insights into the antifungal mechanism of fengycin.
萎蔫病是一种由 引起的世界性土传真菌病害，对农产品造成严重损害。因此，预防和治疗枯萎病具有重要意义。在本研究中，我们从 FAJT-4 中纯化了十种单一的脂肽丰素成分，发现 C 丰素 B 抑制了 FJAT-31362 的生长。我们观察到 C 丰素 B 处理的 细胞中出现了早期细胞凋亡的特征，包括活性氧物质积累、线粒体功能障碍和磷脂酰丝氨酸外翻。进一步的数据表明，C 丰素 B 诱导细胞凋亡依赖于半胱天冬酶。重要的是，我们发现 TOR 信号通路中与自噬相关的基因表达显著上调；同时，酸性自噬空泡在 细胞中的积累表明，在 C 丰素 B 诱导的细胞凋亡过程中，自噬途径被激活。因此，本研究为丰素的抗真菌机制提供了新的见解。
