离子色谱法与中子活化分析法用于微量测定肌肉中钠和钾的比较。

Comparison of ion chromatography and neutron activation analysis for the microdetermination of Na+ and K+ in muscle.

作者信息

Haber E P, Atalla L T

出版信息

Braz J Med Biol Res. 1986;19(1):103-7.

Abstract

The simultaneous determination of Na+ and K+ in small tissue samples by means of neutron activation (NAA) and ion chromatography (IC) is described. A 5 X 10(11) n cm-2 s-1 neutron flux was used for Na+ analysis and the induced 24Na and 42K activities were measured without chemical separation with a Ge(Li) detector coupled to a 4096 channel analyzer. A minicomputer on line with the analyzer was employed for the interpretation of gamma spectra. When IC was used, the sample and the standard were dissolved in the solutions before injection into the ion chromatograph. The ion concentration in the effluent solution was measured by electric conductivity. The two methods were compared in terms of time required, sensitivity, accuracy, precision, simplicity and operational cost of the analysis. Both techniques proved to be excellent from the point of view of the reliability of the results, and can be used for the determination of Na+ and K+ in biological samples.

摘要

本文描述了通过中子活化分析（NAA）和离子色谱法（IC）同时测定小组织样本中的Na⁺和K⁺。使用5×10¹¹ n cm⁻² s⁻¹的中子通量进行Na⁺分析，在不进行化学分离的情况下，用与4096通道分析仪相连的锗（锂）探测器测量诱导产生的²⁴Na和⁴²K活度。使用与分析仪联机的小型计算机来解释γ能谱。当使用离子色谱法时，将样品和标准品溶解在溶液中，然后注入离子色谱仪。通过电导率测量流出溶液中的离子浓度。从分析所需时间、灵敏度、准确度、精密度、简便性和操作成本等方面对这两种方法进行了比较。从结果的可靠性来看，这两种技术都非常出色，可用于测定生物样品中的Na⁺和K⁺。