Rathor Shraddha, Shukla Manisha, Deepak Desh
Department of Chemistry, University of Lucknow, Lucknow, India.
Nat Prod Res. 2025 Apr;39(7):1945-1954. doi: 10.1080/14786419.2023.2284869. Epub 2023 Nov 29.
Buffalo milk processed by method of Kobata and Ginsburg for isolation of its oligosaccharide contents showed significant stimulation of anti-body, delayed type hypersensitivity response to sheep red blood cells in BALB/c mice. This oligosaccharide mixture also stimulated non-specific immune response in terms of MMI. In continuation to our previous studies of oligosaccharide contents of buffalo milk another novel pentasaccharide Ebaliose was isolated from immunostimulant oligosaccharide fraction of buffalo milk. Ebaliose was procured in its purest form, by combining the different chromatographic techniques like Gel filtration, Silica Gel column chromatography, and HPLC. The structure of novel oligosaccharide was determined by H,C, HSQC, TOCSY, COSY, HMBC, ESI-MS, chemical transformation, and chemical degradation. The stereoscopic structure of this novel pentasaccharide was established as under.
采用小幡和金斯伯格方法处理以分离其寡糖成分的水牛奶，对BALB/c小鼠体内抗体、对绵羊红细胞的迟发型超敏反应有显著刺激作用。这种寡糖混合物在巨噬细胞移动抑制方面也刺激了非特异性免疫反应。延续我们之前对水牛奶寡糖成分的研究，从水牛奶的免疫刺激寡糖组分中分离出另一种新型五糖埃巴利糖。通过结合凝胶过滤、硅胶柱色谱和高效液相色谱等不同色谱技术，以最纯的形式获得了埃巴利糖。通过氢谱、碳谱、异核单量子相干谱、全相关谱、同核相关谱、异核多键相关谱、电喷雾电离质谱、化学转化和化学降解确定了新型寡糖的结构。这种新型五糖的立体结构如下所示。
