Saksena R, Deepak D, Khare A, Sahai R, Tripathi L M, Srivastava V M
Department of Chemistry, Lucknow University, Lucknow 226 007, India.
Biochim Biophys Acta. 1999 Aug 5;1428(2-3):433-45. doi: 10.1016/s0304-4165(99)00089-6.
A processed oligosaccharide mixture of buffalo milk induced significant stimulation of antibody, delayed-type hypersensitivity response to sheep red blood cells in BALB/c mice. This also stimulated non-specific immune response of the animals measured in terms of macrophage migration index. A novel pentasaccharide has been isolated from the oligosaccharide containing fraction having immunostimulant activity of buffalo milk. This compound was isolated by a combination of gel filtration chromatography, silica gel column chromatography of derivatised oligosaccharides while the homogeneity was confirmed by high performance liquid chromatography. The results of structural analyses, i.e. proton nuclear magnetic resonance, fast atom bombardment mass spectrometry, chemical transformations and degradations are consistent with the following structure: GlcNAcbeta(1-->3)Galbeta(1-->4)GlcNAcbeta(1-->3)Gal beta(1-->4)Glc
水牛乳的一种加工低聚糖混合物在BALB/c小鼠中显著刺激了抗体，以及对绵羊红细胞的迟发型超敏反应。这也刺激了以巨噬细胞迁移指数衡量的动物非特异性免疫反应。从具有水牛乳免疫刺激活性的低聚糖组分中分离出一种新型五糖。该化合物通过凝胶过滤色谱、衍生化低聚糖的硅胶柱色谱相结合的方法分离得到，同时通过高效液相色谱确认了其纯度。结构分析结果，即质子核磁共振、快原子轰击质谱、化学转化和降解，与以下结构一致：GlcNAcbeta(1-->3)Galbeta(1-->4)GlcNAcbeta(1-->3)Gal beta(1-->4)Glc
