Marano Francelyne
Université Paris Cité, CNRS UMR 8251, Biologie fonctionnelle et adaptative, 4, rue Marie-Andrée Lagroua Weill-Hallé, 75205 Paris cedex 13, France.
Biol Aujourdhui. 2023;217(3-4):199-205. doi: 10.1051/jbio/2023035. Epub 2023 Nov 29.
Alternative methods to animal testing are used in fundamental and clinical research, for the realization of studies for regulatory purposes, and also screening operations in the development of new molecules. They are based on in vitro (cell models) or in silico (mathematical models) replacement methods. They have been largely promoted by the 3Rs rule (Replace, Reduce, Refine) which aims at regulating animal experimentation. For biomedical research, these different methods are valuable tools for better understanding the physiology of organisms and the mechanisms of the effects of chemicals and physical agents on them.
替代动物实验的方法被用于基础研究和临床研究、实现监管目的的研究以及新分子开发中的筛选操作。它们基于体外（细胞模型）或计算机模拟（数学模型）替代方法。这些方法在很大程度上得到了旨在规范动物实验的3R原则（替代、减少、优化）的推动。对于生物医学研究而言，这些不同的方法是宝贵的工具，有助于更好地理解生物体的生理学以及化学和物理因素对其产生影响的机制。
