Department of Biological Sciences, Genetics Program, College of Science, North Carolina State University , Raleigh, North Carolina, USA.
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University , Raleigh, North Carolina, USA.
Microbiol Spectr. 2024 Jan 11;12(1):e0357623. doi: 10.1128/spectrum.03576-23. Epub 2023 Nov 29.
Recent work on bile salt hydrolases (BSHs) in Gram-negative bacteria, such as Bacteroides, has primarily focused on how they can impact host physiology. However, the benefits bile acid metabolism confers to the bacterium that performs it are not well understood. In this study, we set out to define if and how () uses its BSHs and hydroxysteroid dehydrogenase to modify bile acids to provide a fitness advantage for itself and . Genes encoding bile acid-altering enzymes were able to impact how responds to nutrient limitation in the presence of bile acids, specifically carbohydrate metabolism, affecting many polysaccharide utilization loci. This suggests that may be able to shift its metabolism, specifically its ability to target different complex glycans including host mucin, when it comes into contact with specific bile acids in the gut.
最近对革兰氏阴性菌（如拟杆菌属）中胆盐水解酶（BSHs）的研究主要集中在它们如何影响宿主生理学上。然而，执行胆汁酸代谢对细菌本身带来的益处尚不清楚。在这项研究中，我们旨在确定（）是否以及如何利用其 BSH 和羟甾醇脱氢酶来修饰胆汁酸，为自身和提供适应优势。编码改变胆汁酸的酶的基因能够影响在存在胆汁酸的情况下对营养限制的反应，特别是碳水化合物代谢，影响许多多糖利用基因座。这表明，当与肠道中特定的胆汁酸接触时，可能能够改变其代谢，特别是靶向不同复杂聚糖（包括宿主粘蛋白）的能力。
