Nutrition and Clinical Services Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) , Dhaka, Bangladesh.
Centre for Global Child Health, The Hospital for Sick Children , Toronto, Ontario, Canada.
Microbiol Spectr. 2024 Jan 11;12(1):e0271123. doi: 10.1128/spectrum.02711-23. Epub 2023 Nov 29.
When administered for seven consecutive days shortly after birth, the probiotic bacterium ATCC 202195 plus fructooligosaccharide (FOS) was reported to reduce sepsis and lower respiratory tract infection events during early infancy in a randomized trial in India. Since probiotic effects are often strain specific, strain-level detection and quantification by routine molecular methods enables the monitoring of safety outcomes, such as probiotic-associated bacteremia, and allows for the quality of probiotic interventions to be assessed and monitored (i.e., verify strain identity and enumerate). Despite the potential clinical applications of ATCC 202195, an assay to detect and quantify this strain has not previously been described. Herein, we report the design of primer and probe sequences to detect ATCC 202195 and the development and optimization of a real-time PCR assay to detect and quantify the strain with high specificity and high sensitivity.
当在出生后连续 7 天内给予时，在印度的一项随机试验中，报道了益生菌细菌 ATCC 202195 加低聚果糖（FOS）可减少婴儿早期的败血症和下呼吸道感染事件。由于益生菌的作用通常是菌株特异性的，因此常规分子方法的菌株水平检测和定量可以监测安全性结果，例如益生菌相关菌血症，并可以评估和监测益生菌干预措施的质量（即，验证菌株身份并计数）。尽管 ATCC 202195 具有潜在的临床应用，但以前尚未描述检测和定量该菌株的方法。在这里，我们报告了设计用于检测 ATCC 202195 的引物和探针序列，并开发和优化了一种实时 PCR 检测和定量该菌株的方法，具有很高的特异性和灵敏度。
