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基于高通量富集的基因组测序技术支持甲型肝炎病毒爆发的调查。

High-performance enrichment-based genome sequencing to support the investigation of hepatitis A virus outbreaks.

机构信息

The Center for Pathogen Genomics, The University of Melbourne , Melbourne, Victoria, Australia.

Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity , Melbourne, Victoria, Australia.

出版信息

Microbiol Spectr. 2024 Jan 11;12(1):e0283423. doi: 10.1128/spectrum.02834-23. Epub 2023 Nov 29.

DOI:10.1128/spectrum.02834-23
PMID:38018979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10783085/
Abstract

This proof-of-concept study introduces a hybrid capture oligo panel for whole-genome sequencing of all six human pathogenic hepatitis A virus (HAV) subgenotypes, exhibiting a higher sensitivity than some conventional genotyping assays. The ability of hybrid capture to enrich multiple targets allows for a single, streamlined workflow, thus facilitating the potential harmonization of molecular surveillance of HAV with other enteric viruses. Even challenging sample matrices can be accommodated, making them suitable for broad implementation in clinical and public health laboratories. This innovative approach has significant implications for enhancing multijurisdictional outbreak investigations as well as our understanding of the global diversity and transmission dynamics of HAV.

摘要

本概念验证研究介绍了一种用于全基因组测序的混合捕获寡核苷酸面板，可对所有六种人类致病性甲型肝炎病毒（HAV）亚型进行测序，其灵敏度高于一些传统的基因分型检测方法。杂交捕获富集多个靶标的能力可实现单一、简化的工作流程，从而促进 HAV 与其他肠道病毒的分子监测的潜在协调。即使是具有挑战性的样本基质也可以适应，使其适合在临床和公共卫生实验室中广泛实施。这种创新方法对于增强多司法管辖区暴发调查以及我们对 HAV 的全球多样性和传播动态的理解具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/725f1749a3ee/spectrum.02834-23.f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/56ff0b04b8f6/spectrum.02834-23.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/f902b7df4036/spectrum.02834-23.f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/d3b89b24806b/spectrum.02834-23.f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/960946350983/spectrum.02834-23.f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/725f1749a3ee/spectrum.02834-23.f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/56ff0b04b8f6/spectrum.02834-23.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/f902b7df4036/spectrum.02834-23.f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/d3b89b24806b/spectrum.02834-23.f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/960946350983/spectrum.02834-23.f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ad4/10783085/725f1749a3ee/spectrum.02834-23.f005.jpg

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