Center for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA.
Hackensack Meridian School of Medicine , Nutley, New Jersey, USA.
Microbiol Spectr. 2024 Jan 11;12(1):e0369223. doi: 10.1128/spectrum.03692-23. Epub 2023 Nov 29.
Understanding the role of IncF plasmids in the success of drug-resistant bacteria has far-reaching implications for tackling antibiotic resistance. The study's use of a novel CRISPR-Cas9-mediated plasmid-curing system provides a precision tool for dissecting the specific impact of IncF plasmids on ExPEC clones, especially high-risk, multidrug-resistant strains like ST131, ST1193, and ST410. The study offers a crucial stepping stone for future research into understanding how these plasmids influence more complex aspects of bacterial behavior, such as cell invasion and fitness.
了解 IncF 质粒在耐药菌成功中的作用，对解决抗生素耐药性问题具有深远的意义。该研究使用新型 CRISPR-Cas9 介导的质粒消除系统，为剖析 IncF 质粒对 ExPEC 克隆，特别是 ST131、ST1193 和 ST410 等高危、多药耐药株的具体影响提供了精确工具。该研究为未来研究这些质粒如何影响细菌行为更复杂的方面，如细胞入侵和适应性，提供了重要的基石。
