Department of Biochemistry, Duke University Medical Center, Durham, NC, USA.
Department of Neurobiology, Duke University Medical Center, Durham, NC, USA.
Methods Mol Biol. 2024;2728:99-104. doi: 10.1007/978-1-0716-3495-0_8.
Trophoblast fusion or syncytialization is a fundamental yet poorly understood process during placental development. Primary cultured cytotrophoblasts and human choriocarcinoma cell lines are commonly used to study trophoblast fusion mechanisms in vitro. Quantification of trophoblast fusion index is a key for the in vitro studies. In this chapter, we describe a new method to quantify fusion index, which is based on fluorescent labeling of the plasma membrane using Di-8-ANEPPS, a membrane potential dye. This method directly works on live cells, thereby is simple, economic, and reliable.
滋养层融合或合胞体化是胎盘发育过程中一个基本但尚未被充分理解的过程。原代培养的滋养层细胞和人绒毛膜癌细胞系常用于体外研究滋养层融合机制。滋养层融合指数的定量是体外研究的关键。在本章中，我们描述了一种新的方法来定量融合指数，该方法基于使用 Di-8-ANEPPS 对质膜进行荧光标记，Di-8-ANEPPS 是一种膜电位染料。该方法直接作用于活细胞，因此简单、经济、可靠。
