Kumar Harender, Garg Vincy, Kaur Kawalpreet, Kaur Ravneet
Department of Zoology, Panjab University, Chandigarh, Punjab, India.
Department of Botany, SGGS College, Chandigarh, Punjab, India.
Ann Neurosci. 2023 Oct;30(4):236-241. doi: 10.1177/09727531231166202. Epub 2023 Apr 7.
Stress can affect the morphology and synaptic organization of the telencephalon. These structural changes at the cellular level can lead to the development of various psychopathologies.
Given that the telencephalon plays a major role in stress responses, the current study aimed to investigate the role of as a neuroprotectant supplement in the early life of zebrafish in averting the alteration of synapse morphology in the telencephalon caused by chronic unpredictable stress (CUS) in the later stage.
5dpf larvae were divided into two groups: one group was fed with a commercial fish diet and a second group with a 1% Spirulina-supplemented diet for 90 days. After 90 days, the adult zebrafish were exposed to CUS with different chronic stressors for 15 days. The synaptic plasticity was evaluated by morphometric analysis of synapse in telencephalon of zebrafish by transmission electron microscopy.
The ultrastructural study demonstrated the protective role of Spirulina in the CUS model as no significant alterations in the length of the active zone, postsynaptic density, and synaptic cleft were observed as compared to the control group in the CUS model.
Thus, suggesting that the Spirulina supplementation can avert the remodeling effect of stress on synapse ultrastructure.
应激可影响端脑的形态和突触组织。这些细胞水平的结构变化可导致各种精神病理学的发展。
鉴于端脑在应激反应中起主要作用，本研究旨在探讨作为一种神经保护补充剂在斑马鱼早期生活中对避免后期慢性不可预测应激（CUS）引起的端脑突触形态改变的作用。
将5日龄的幼虫分为两组：一组喂食商业鱼饲料，另一组喂食添加1%螺旋藻的饲料，持续90天。90天后，成年斑马鱼暴露于不同慢性应激源的CUS中15天。通过透射电子显微镜对斑马鱼端脑突触进行形态计量分析来评估突触可塑性。
超微结构研究表明螺旋藻在CUS模型中具有保护作用，与CUS模型中的对照组相比，活性区长度、突触后密度和突触间隙均未观察到显著变化。
因此，表明补充螺旋藻可避免应激对突触超微结构的重塑作用。
