Subba Rao G, Hanjani P
Cancer Lett. 1986 Dec;33(3):325-32. doi: 10.1016/0304-3835(86)90072-8.
The epithelial cells from both control and neoplastic ovaries were grown as primary cultures. The outgrowth of epithelial cells occurred within 3-5 days and the cells formed essentially a monolayer culture covering more than 80% of the surface of the flask within 5 weeks. The incorporation of [3H]fucose into the glycoproteins secreted into the medium was measured in the cells grown for 4 weeks. The secretion of glycoproteins in the tumor cells was twice that of the control. Analysis of the glycoproteins in the medium showed that the ovarian epithelial tumor cells secreted predominantly a 97 kilodalton glycoprotein. The growth of fibroblasts could be inhibited in these cultures by using Falcon Primaria culture flasks and the growth medium containing D-valine.
来自对照卵巢和肿瘤性卵巢的上皮细胞作为原代培养物进行培养。上皮细胞在3 - 5天内开始生长，并且在5周内细胞基本上形成覆盖培养瓶表面80%以上的单层培养物。在培养4周的细胞中测量了[3H]岩藻糖掺入分泌到培养基中的糖蛋白的情况。肿瘤细胞中糖蛋白的分泌是对照细胞的两倍。对培养基中糖蛋白的分析表明，卵巢上皮肿瘤细胞主要分泌一种97千道尔顿的糖蛋白。通过使用Falcon Primaria培养瓶和含有D - 缬氨酸的生长培养基，可以在这些培养物中抑制成纤维细胞的生长。
