Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Hamburg, Germany.
Mol Microbiol. 2024 Apr;121(4):646-658. doi: 10.1111/mmi.15203. Epub 2023 Dec 1.
Super-resolution fluorescence microscopy technologies developed over the past two decades have pushed the resolution limit for fluorescently labeled molecules into the nanometer range. These technologies have the potential to study bacterial structures, for example, macromolecular assemblies such as secretion systems, with single-molecule resolution on a millisecond time scale. Here we review recent applications of super-resolution fluorescence microscopy with a focus on bacterial secretion systems. We also describe MINFLUX fluorescence nanoscopy, a relatively new technique that promises to one day produce molecular movies of molecular machines in action.
过去二十年发展起来的超分辨率荧光显微镜技术已经将荧光标记分子的分辨率推到了纳米级。这些技术有可能在毫秒时间尺度上以单分子分辨率研究细菌结构，例如分泌系统等大分子组装体。在这里，我们回顾了超分辨率荧光显微镜的最新应用，重点介绍了细菌分泌系统。我们还描述了 MINFLUX 荧光纳米显微镜，这是一种相对较新的技术，有望在未来制作出分子机器在工作时的分子电影。
