Tubbs R R, Velasco M E, Benjamin S P
Arch Pathol Lab Med. 1979 Sep;103(10):534-6.
An immunomicroscopic study evaluated 55 fixed, paraffin-embedded tissues from surgical and necropsy-accessioned material reflecting a variety of physiologic and pathologic disorders. The sensitive unlabeled (peroxidase-antiperoxidase) immunoperoxidase technique was used. Cytoplasmic human chorionic gonadotropin (CHCG) was readily identified in syncytial trophoblastic cells. The only tissue sections that did not demonstrate CHCG activity were primary and secondary testicular teratocarcinomas without choriocarcinoma components. When the technique was reevaluated for the non-hazardous chromogen 3-amino, 9-ethylcarbazole, excellent results were achieved with equivalent staining distribution and intensity. The unlabeled immunoperoxidase method for the identification of CHCG is a sensitive, reliable, confirmatory immunohistochemical stain. A relatively safe chromogen can be substituted for the benzidine derivatives in the procedure with equivalent results.
一项免疫显微镜研究评估了55份来自手术和尸检材料的固定石蜡包埋组织，这些组织反映了各种生理和病理紊乱情况。采用了敏感的未标记（过氧化物酶-抗过氧化物酶）免疫过氧化物酶技术。在合体滋养层细胞中很容易鉴定出细胞质人绒毛膜促性腺激素（CHCG）。唯一未显示CHCG活性的组织切片是没有绒毛膜癌成分的原发性和继发性睾丸畸胎癌。当对非危险性显色剂3-氨基-9-乙基咔唑重新评估该技术时，获得了优异的结果，染色分布和强度相当。用于鉴定CHCG的未标记免疫过氧化物酶方法是一种敏感、可靠的验证性免疫组织化学染色。在该程序中，可以用一种相对安全的显色剂替代联苯胺衍生物，结果相同。
