Tobajas Yaiza, Alemany-Fornés Marc, Samarra Iris, Romero-Giménez Jordi, Tintoré Maria, Del Pino Antoni, Canela Núria, Del Bas Josep M, Ortega-Olivé Nàdia, de Lecea Carlos, Escoté Xavier
EURECAT, Centre Tecnològic de Catalunya, Nutrition and Health, 43204 Reus, Spain.
DR Healthcare-AB Biotek HNH, 08017 Barcelona, Spain.
J Clin Med. 2023 Dec 4;12(23):7502. doi: 10.3390/jcm12237502.
Histamine intolerance arises when there is a disparity between the production of histamine and the body's ability to break it down. In the gastrointestinal tract, the primary enzyme responsible for metabolizing ingested histamine is diamine oxidase (DAO), and a shortage of this enzyme has been associated with some diseases related to the respiratory, cardiovascular, nervous, muscular, and digestive systems, in addition to migraines. The treatment of migraines typically revolves around the utilization of both anti-migraine and anti-inflammatory drugs, but their interaction with DAO is not thoroughly understood. In this study, we examined the impact of nonsteroidal anti-inflammatory drugs (NSAIDs) and anti-migraine medications on DAO activity through in vitro experiments. We also investigated their effects on the human intestinal cell line Caco-2, assessing changes in DAO expression (both at the mRNA and protein levels) as well as DAO activity. The tested drugs, including ibuprofen, acetylsalicylic acid, paracetamol, a combination of acetylsalicylic acid with paracetamol and caffeine, zolmitriptan, and sumatriptan, did not inhibit DAO activity or reduce their levels. However, naproxen reduced DAO protein levels in human enterocyte cultures while not affecting DAO activity. These results suggest that combining anti-inflammatory and anti-migraine drugs with DAO enzyme supplementation for migraine patients with DAO deficiency could be beneficial for healthcare professionals in their daily practice.
当组胺的产生与身体分解组胺的能力之间存在差异时，就会出现组胺不耐受。在胃肠道中，负责代谢摄入组胺的主要酶是二胺氧化酶（DAO），这种酶的缺乏与一些呼吸系统、心血管系统、神经系统、肌肉系统和消化系统疾病以及偏头痛有关。偏头痛的治疗通常围绕使用抗偏头痛和抗炎药物展开，但它们与DAO的相互作用尚未完全了解。在本研究中，我们通过体外实验研究了非甾体抗炎药（NSAIDs）和抗偏头痛药物对DAO活性的影响。我们还研究了它们对人肠道细胞系Caco-2的影响，评估了DAO表达（mRNA和蛋白质水平）以及DAO活性的变化。所测试的药物，包括布洛芬、乙酰水杨酸、对乙酰氨基酚、乙酰水杨酸与对乙酰氨基酚和咖啡因的组合、佐米曲普坦和舒马曲普坦，均未抑制DAO活性或降低其水平。然而，萘普生降低了人肠细胞培养物中DAO的蛋白质水平，而不影响DAO活性。这些结果表明，对于DAO缺乏的偏头痛患者，将抗炎和抗偏头痛药物与DAO酶补充剂联合使用，可能对医疗专业人员的日常实践有益。
