从高海拔药用植物佩雷齐亚·科雷乌莱塞斯中分离出的短小青霉 HE19ct 的多种生物活性和次生代谢产物特征。

Diverse biological activities and secondary metabolites profile of Penicillium brevicompactum HE19ct isolated from the high-Andean medicinal plant Perezia coerulescens.

机构信息

Centro de Investigación de La Biodiversidad y Recursos Genéticos, Universidad Nacional Santiago Antúnez de Mayolo, Av. Centenario 200, 02002, Peru.

出版信息

Fungal Biol. 2023 Dec;127(12):1439-1450. doi: 10.1016/j.funbio.2023.10.002. Epub 2023 Nov 7.

DOI:10.1016/j.funbio.2023.10.002

PMID:38097318

Abstract

Endophytic fungi produced attractive primary and secondary metabolites for industries, pharmacology, and biotechnology. The bioactive potential of HE19ct, identified as Penicillium brevicompactum according to ITS-BenA-caM, was addressed. Antimicrobial and antioxidant activities and secondary metabolite contents using four culture media in Agar-plate (ApF) and Submerged (SmF) fermentation were evaluated. Some plant growth-promoting (PGP) traits and their related genes were tested. HE19ct exhibited antimicrobial activity against Staphylococcus aureus, Enterococcus faecalis, Candida albicans, C. tropicalis, Fusarium sp., Geotrichum candidum, and Alternaria sp. All cultures showed DPPH scavenging activity and phenolic compounds, where ethyl acetate extract of SmF with malt extract showed higher activity and SmF/ApF with potato-dextrose exhibited higher yield, respectively. HE19ct solubilized tricalcium-phosphate and produced siderophore, endoglucanase, proteinase, and amylase. It enhanced the alfalfa's germination at 15 °C, root development, and phenols production at 15 and 24 °C. Phenols, tannins, anthraquinones, triterpenoids/steroids, and alkaloids production were detected depending on culture media. Polyketide synthase type I gene (PksI), subtilisin-like protease prb 1 (Pbr), and siderophore D (sidD) were PCR-amplified. Finally, HE19CT could be a promising source of interesting bioactive compounds for pharmacology and agriculture mainly in extreme conditions, then metabolomic and functional genetic research must be performed to support their appropriate application.

摘要

内生真菌产生有吸引力的初级和次级代谢产物，用于工业、药理学和生物技术。根据 ITS-BenA-caM，鉴定 HE19ct 为短密青霉，研究了其生物活性潜力。采用琼脂平板（ApF）和深层发酵（SmF）两种培养方法，评估了四种培养基中的抗菌和抗氧化活性以及次生代谢产物含量。测试了一些植物促生（PGP）特性及其相关基因。HE19ct 对金黄色葡萄球菌、粪肠球菌、白色念珠菌、热带念珠菌、镰刀菌、近平滑念珠菌和链格孢菌表现出抗菌活性。所有培养物均显示出 DPPH 清除活性和酚类化合物，其中 SmF 与麦芽提取物的乙酸乙酯提取物表现出更高的活性，SmF/ApF 与马铃薯-葡萄糖表现出更高的产率。HE19ct 可溶解磷酸三钙并产生铁载体、内切葡聚糖酶、蛋白酶和淀粉酶。它在 15°C 下促进了紫花苜蓿的发芽、根的发育和在 15 和 24°C 下的酚类物质的产生。根据培养基的不同，检测到了酚类、单宁、蒽醌、三萜/甾体和生物碱的产生。PCR 扩增了聚酮合酶 I 型基因（PksI）、枯草杆菌蛋白酶类似物 prb1（Pbr）和铁载体 D（sidD）。最后，HE19CT 可能是药理学和农业中具有有趣生物活性化合物的有前途的来源，特别是在极端条件下，然后必须进行代谢组学和功能遗传研究，以支持其适当的应用。